p-Phenylenediamine induces p53-mediated apoptosis in Mardin-Darby canine kidney cells

Ssu Ching Chen; Ching Hsein Chen; Chi Liang Chern; Li Sung Hsu; Ya Chun Huang; King Thom Chung; Soi Moi Chye

doi:10.1016/j.tiv.2005.12.003

p-Phenylenediamine induces p53-mediated apoptosis in Mardin-Darby canine kidney cells

Ssu Ching Chen, Ching Hsein Chen, Chi Liang Chern, Li Sung Hsu, Ya Chun Huang, King Thom Chung, Soi Moi Chye

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The mechanism of toxicity p-phenylenediamine (p-PD), a component of human permanent hair dye and a suspected carcinogen, on the growth of Mardin-Darby canine kidney cells (MDCK) was investigated. With the analysis of flow cytometry, a dose-dependent accumulation of the sub-G1 peak and the G0/G1-phase arrested in cell cycle, and time-dependent induction of apoptosis after staining with Annexin V-Fluorescein and propidium iodide were observed. After the treatment of cells with p-PD, dose dependent DNA fragmentation shown by gel electrophoresis, the reduction of membrane potential (ΔΨ_m) by mitochondria membrane depolarization and the increase of the expression of p53 protein in cells, suggested that the effect of p-PD on overall viability and cell numbers is mediated by an increase in apoptosis.

Original language	English
Pages (from-to)	801-807
Number of pages	7
Journal	Toxicology in Vitro
Volume	20
Issue number	6
DOIs	https://doi.org/10.1016/j.tiv.2005.12.003
State	Published - Sep 2006

Keywords

Apoptosis
Mardin-Darby canine kidney cells
p-Phenylenediamine
p53

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title = "p-Phenylenediamine induces p53-mediated apoptosis in Mardin-Darby canine kidney cells",

abstract = "The mechanism of toxicity p-phenylenediamine (p-PD), a component of human permanent hair dye and a suspected carcinogen, on the growth of Mardin-Darby canine kidney cells (MDCK) was investigated. With the analysis of flow cytometry, a dose-dependent accumulation of the sub-G1 peak and the G0/G1-phase arrested in cell cycle, and time-dependent induction of apoptosis after staining with Annexin V-Fluorescein and propidium iodide were observed. After the treatment of cells with p-PD, dose dependent DNA fragmentation shown by gel electrophoresis, the reduction of membrane potential (ΔΨm) by mitochondria membrane depolarization and the increase of the expression of p53 protein in cells, suggested that the effect of p-PD on overall viability and cell numbers is mediated by an increase in apoptosis.",

keywords = "Apoptosis, Mardin-Darby canine kidney cells, p-Phenylenediamine, p53",

author = "Chen, {Ssu Ching} and Chen, {Ching Hsein} and Chern, {Chi Liang} and Hsu, {Li Sung} and Huang, {Ya Chun} and Chung, {King Thom} and Chye, {Soi Moi}",

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T1 - p-Phenylenediamine induces p53-mediated apoptosis in Mardin-Darby canine kidney cells

AU - Chen, Ssu Ching

AU - Chen, Ching Hsein

AU - Chern, Chi Liang

AU - Hsu, Li Sung

AU - Huang, Ya Chun

AU - Chung, King Thom

AU - Chye, Soi Moi

PY - 2006/9

Y1 - 2006/9

N2 - The mechanism of toxicity p-phenylenediamine (p-PD), a component of human permanent hair dye and a suspected carcinogen, on the growth of Mardin-Darby canine kidney cells (MDCK) was investigated. With the analysis of flow cytometry, a dose-dependent accumulation of the sub-G1 peak and the G0/G1-phase arrested in cell cycle, and time-dependent induction of apoptosis after staining with Annexin V-Fluorescein and propidium iodide were observed. After the treatment of cells with p-PD, dose dependent DNA fragmentation shown by gel electrophoresis, the reduction of membrane potential (ΔΨm) by mitochondria membrane depolarization and the increase of the expression of p53 protein in cells, suggested that the effect of p-PD on overall viability and cell numbers is mediated by an increase in apoptosis.

AB - The mechanism of toxicity p-phenylenediamine (p-PD), a component of human permanent hair dye and a suspected carcinogen, on the growth of Mardin-Darby canine kidney cells (MDCK) was investigated. With the analysis of flow cytometry, a dose-dependent accumulation of the sub-G1 peak and the G0/G1-phase arrested in cell cycle, and time-dependent induction of apoptosis after staining with Annexin V-Fluorescein and propidium iodide were observed. After the treatment of cells with p-PD, dose dependent DNA fragmentation shown by gel electrophoresis, the reduction of membrane potential (ΔΨm) by mitochondria membrane depolarization and the increase of the expression of p53 protein in cells, suggested that the effect of p-PD on overall viability and cell numbers is mediated by an increase in apoptosis.

KW - Apoptosis

KW - Mardin-Darby canine kidney cells

KW - p-Phenylenediamine

KW - p53

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U2 - 10.1016/j.tiv.2005.12.003

DO - 10.1016/j.tiv.2005.12.003

M3 - 期刊論文

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AN - SCOPUS:33745187571

SN - 0887-2333

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SP - 801

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JO - Toxicology in Vitro

JF - Toxicology in Vitro

IS - 6

ER -

p-Phenylenediamine induces p53-mediated apoptosis in Mardin-Darby canine kidney cells

Abstract

Keywords

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