Optimizing the 13C-14N REAPDOR NMR Experiment: A Theoretical and Experimental Study

Yong Ba, Hsien Ming Kao, Clare P. Grey, Lamy Chopin, Terry Gullion

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58 Scopus citations

Abstract

The optimum 14N pulse lengths in the 13C-14N rotational-echo adiabatic-passage double-resonance (REAPDOR) NMR experiment are determined from calculations and from experiments on samples of glycine and L-alanine. The REAPDOR experiment utilizes the adiabatic passages that 14N spins make between the 14N Zeeman energy levels during the application of a single, short 14N radiofrequency pulse. Use of a short 14N irradiation time of less than one-quarter of a rotor period ensures that the number of 14N spins that undergo more than one passage is minimized. This simplifies calculations describing 13C dipolar dephasing and provides better agreement between calculations and experiments. Recovery of the 13C-14N dipolar couplings and 14N quadrupolar coupling constants and asymmetry parameters is described.

Original languageEnglish
Pages (from-to)104-114
Number of pages11
JournalJournal of Magnetic Resonance
Volume133
Issue number1
DOIs
StatePublished - Jul 1998

Keywords

  • Double resonance
  • Nitrogen
  • NMR
  • REAPDOR

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