On-line SERS detection of single bacterium Using Novel SERS Nanoprobes and a microfl uidic Dielectrophoresis device

Hsing Ying Lin, Chen Han Huang, Wen Hsin Hsieh, Ling Hsuan Liu, Yuan Chuen Lin, Chia Chun Chu, Shi Ting Wang, I. Ting Kuo, Lai Kwan Chau, Chiou Ying Yang

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

The integration of novel surface-enhanced Raman scattering (SERS) nanoprobes and a microfl uidic dielectrophoresis (DEP) device is developed for rapid on-line SERS detection of Salmonella enterica serotype Choleraesuis and Neisseria lactamica . The SERS nanoprobes are prepared by immobilization of specifi c antibody onto the surface of nanoaggregate-embedded beads (NAEBs), which are silica-coated, dye-induced aggregates of a small number of gold nanoparticles (AuNPs). Each NAEB gives highly enhanced Raman signals owing to the presence of well-defi ned plasmonic hot spots at junctions between AuNPs. Herein, the on-line SERS detection and accurate identifi cation of suspended bacteria with a detection capability down to a single bacterium has been realized by the NAEB?DEP?Raman spectroscopy biosensing strategy. The practical detection limit with a measurement time of 10 min is estimated to be 70 CFU mL ?1 . In comparison with whole-cell enzyme-linked immunosorbent assay (ELISA), the SERS-nanoprobe-based biosensing method provides advantages of higher sensitivity and requiring lower amount of antibody in the assay (100-fold less). The total assay time including sample pretreatment is less than 2 h. Hence, this sensing strategy is promising for faster and effective on-line multiplex detection of single pathogenic bacterium by using different bioconjugated SERS nanoprobes.

Original languageEnglish
Pages (from-to)4700-4710
Number of pages11
JournalSmall
Volume10
Issue number22
DOIs
StatePublished - 26 Nov 2014

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