Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival

Sue Jane Lin; Shao Wen Wu; Ya Ching Chou; Jiun Han Lin; Ya Chi Huang; Mei Ru Chen; Nianhan Ma; Ching Hwa Tsai

doi:10.1016/j.virol.2015.02.015

Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival

Sue Jane Lin, Shao Wen Wu, Ya Ching Chou, Jiun Han Lin, Ya Chi Huang, Mei Ru Chen, Nianhan Ma, Ching Hwa Tsai

Department of Biomedical Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.

Original language	English
Pages (from-to)	24-33
Number of pages	10
Journal	Virology
Volume	481
DOIs	https://doi.org/10.1016/j.virol.2015.02.015
State	Published - 1 Jul 2015

Keywords

Apoptosis
EBV
LCL
TIMP-1
Zta

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title = "Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival",

abstract = "Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.",

keywords = "Apoptosis, EBV, LCL, TIMP-1, Zta",

author = "Lin, {Sue Jane} and Wu, {Shao Wen} and Chou, {Ya Ching} and Lin, {Jiun Han} and Huang, {Ya Chi} and Chen, {Mei Ru} and Nianhan Ma and Tsai, {Ching Hwa}",

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T1 - Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival

AU - Lin, Sue Jane

AU - Wu, Shao Wen

AU - Chou, Ya Ching

AU - Lin, Jiun Han

AU - Huang, Ya Chi

AU - Chen, Mei Ru

AU - Ma, Nianhan

AU - Tsai, Ching Hwa

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.

AB - Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.

KW - Apoptosis

KW - EBV

KW - LCL

KW - TIMP-1

KW - Zta

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U2 - 10.1016/j.virol.2015.02.015

DO - 10.1016/j.virol.2015.02.015

M3 - 期刊論文

C2 - 25765004

AN - SCOPUS:84924296960

SN - 0042-6822

VL - 481

SP - 24

EP - 33

JO - Virology

JF - Virology

ER -

Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival

Abstract

Keywords

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