Non-de-scanned parallel recording two-photon hyperspectral microscopy with high spectral and spatial resolution

Szu Yu Chen, Chiao Sheng Lu, Chia Hua Yeh

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A two-photon hyperspectral microscope with non-de-scanned geometry and parallel recording scheme was constructed which had a low dwell time, high spectral resolution, and high spatial resolution. The dwell time was effectively decreased to reduce photodamage through parallel recording, while the non-de-scanned geometry led to a considerable reduction in the signal loss and spectrum distortion. Two experiments were performed to show that this system can solve crosstalk issues and spectrally resolve the intrinsic fluorophores in optically-thick tissues without staining and sectioning.

Original languageEnglish
Pages (from-to)338-347
Number of pages10
JournalBiomedical Optics Express
Volume5
Issue number2
DOIs
StatePublished - 2014

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