Abstract
A two-photon hyperspectral microscope with non-de-scanned geometry and parallel recording scheme was constructed which had a low dwell time, high spectral resolution, and high spatial resolution. The dwell time was effectively decreased to reduce photodamage through parallel recording, while the non-de-scanned geometry led to a considerable reduction in the signal loss and spectrum distortion. Two experiments were performed to show that this system can solve crosstalk issues and spectrally resolve the intrinsic fluorophores in optically-thick tissues without staining and sectioning.
| Original language | English |
|---|---|
| Pages (from-to) | 338-347 |
| Number of pages | 10 |
| Journal | Biomedical Optics Express |
| Volume | 5 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2014 |