Nanoparticle based simple electrochemical biosensor platform for profiling of protein-nucleic acid interactions

Yifan Dai, Liang Yuan Chiu, Yongkun Sui, Quanbin Dai, Srinivasa Penumutchu, Niyati Jain, Liming Dai, Christian A. Zorman, Blanton S. Tolbert, R. Mohan Sankaran, Chung Chiun Liu

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The analysis of protein-nucleic acid interactions is essential for biophysics related research. However, simple, rapid, and accurate methods for quantitative analysis of biomolecular interactions are lacking. We herein establish an electrochemical biosensor approach for protein-nucleic acid binding analysis. Nanoparticle based sensors are fabricated by highly-controlled inkjet printing followed by plasma conversion. A novel bioconjugation method is demonstrated as a simple and rapid approach for protein-based biosensor fabrication. As a proof of concept, we analyzed the binding interaction between unwinding protein 1 (UP1) and SL3ESS3 RNA, confirming the accuracy of this nanoparticle based electrochemical biosensor approach with traditional biophysical methods. We further accurately profiled and differentiated a unique binding interaction pattern of multiple G-tract nucleic acid sequences with heterogeneous nuclear ribonucleoprotein H1. Our study provides insights into a potentially universal platform for in vitro biomolecule interaction analysis using a nanoparticle based electrochemical biosensor approach.

Original languageEnglish
Pages (from-to)46-54
Number of pages9
JournalTalanta
Volume195
DOIs
StatePublished - 1 Apr 2019

Keywords

  • G-tract nucleic acid
  • Heterogeneous nuclear ribonucleoprotein H1
  • Microsystem for binding interaction analysis
  • Nanoparticle electrochemical biosensor
  • Plasma

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