Nanometer imaging by differential confocal microscopy and its applications in biology

Chau Hwang Lee, Jyhpyng Wang, Chin Lin Guo

Research output: Contribution to journalConference articlepeer-review

Abstract

By utilizing the sharp slopes of the confocal response function, we developed a novel optical technique - differential confocal microscopy - for nanometer surface imaging. With a simple set-up, a 2 nm depth resolution has been demonstrated. Neither servo feedback loop nor lock-in detection are used, hence the technique can be used for high-speed surface profiling at long working distance. Because the technique uses no interferometric effects, it offers large open-loop dynamic range and is compatible with fluorescence microscopy. These features make it ideal for real-time monitoring cell membrane motion in natural environments. Using this technique we measured the dynamic viscoelastic and biochemical responses of living cells to sub-micrometer laser-driven deformation. Cytoplasmic gel-to-sol transition was detected with high sensitivity, and its influence on the viscoelastic response and Ca2+ release was studied.

Original languageEnglish
Pages (from-to)1715-1718
Number of pages4
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume4
StatePublished - 1998
EventProceedings of the 1998 20th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 4 (of 6) - Hong Kong, China
Duration: 29 Oct 19981 Nov 1998

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