Nanofilament Silicon for Matrix-Free Laser Desorption/Ionization Mass Spectrometry

Chia Wen Tsao, Don L. DeVoe

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations


Mass spectrometry (MS) provides quantitative information toward accurate mass identification of unknown molecules and has become a powerful and widely used technique for bioanalysis. In this chapter, we describe the fabrication and MS analysis steps for a high sensitivity matrix-free laser desorption/ionization mass spectrometry (LDI-MS) target substrate termed nanofilament silicon (nSi). Unlike other nanostructured porous silicon surfaces, nSi possesses an open pore morphology with associated benefits including efficient transport of sample into the nanopores and effective removal of solvent from the surface. The utility of nSi targets for LDI-MS analysis lies primarily in the analysis of small molecules, which are typically less than 5,000 Da with a detection sensitivity in the attomole range.

Original languageEnglish
Title of host publicationNanoproteomics
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages7
ISBN (Print)9781617793189
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Desorption/ionization on silicon
  • Mass spectrometry
  • Matrix-free laser desorption/ionization
  • Nanofilament silicon
  • Nanostructured materials


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