Multiplex fiber-optic biosensor using multiple particle plasmon resonances

Hsing Ying Lin, Chen Han Huang, Yu Chia Liu, Kuo Wei Huang, Lai Kwan Chau

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations


Multiplex fiber-optic biosensor implemented by integrating multiple particle plasmon resonances (PPRs), molecular bioassays, and microfluidics is successfully demonstrated. The multiple PPRs are achieved by chemical immobilization of silver nanoparticles (AgNPs) and gold nanorods (AuNRs) separately on two unclad portions of an optical fiber. The difference in morphology and nature of material of AgNPs and AuNRs are exploited to yield multiple plasmonic absorptions at 405 and 780 nm in the absorption spectrum measured from optical fiber by white light source illumination. Through the coaxial excitation of light-emitting diodes (LEDs) with 405 and 800 nm wavelengths, the distinct PPRs are advantageous for real-time and simultaneous detection of multiple analyte-probe pairs as AgNPs and AuNRs are separately functionalized with specific bio-probes. Here, the multi-window fiber-optic particle plasmon resonance (FO-PPR) biosensor has been shown to be capable of simultaneously detecting anti-dinitrophenyl antibody (anti-DNP, MW = 220 kDa) via N-(2,4-dinitrophenyl)-6-aminohexanoic acid (DNP, MW = 297.27 Da) functionalized AgNPs and streptavidin (MW = 75 kDa) via N-(3-aminopropyl) biotinamide trifluoroacetate (biotin, MW = 414.44 Da) functionalized AuNRs. The multiplex sensing chip possesses several advantages, including rapid and parallel detection of multiple analytes on a single chip, minimized sample to sample variation, reduced amount of sensor chip, and reduced analyte volume, hence it is ideally suitable for high-throughput multiplex biochemical sensing applications.

Original languageEnglish
Title of host publicationThird Asia Pacific Optical Sensors Conference
StatePublished - 2012
Event3rd Asia Pacific Optical Sensors Conference - Sydney, NSW, Australia
Duration: 31 Jan 20123 Feb 2012

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X


Conference3rd Asia Pacific Optical Sensors Conference
CitySydney, NSW


  • affinity biosensor
  • fiber optic sensor
  • gold nanorods
  • localized surface plasmon resonance
  • multi-analyte biosensing
  • multiplex bioassays
  • particle plasmon resonance
  • silver nanoparticles


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