Projects per year
Abstract
The activation of peroxisome proliferator-activated rece ptor gamma (PPAR-γ) is known to induce the differentiation of adipocytes. This study aimed to investigate the probiotic effect of Leuconostoc mesenteroides (L. mesenteroides) on high-fat diet (HFD)-induced PPAR-γ activation and abdominal fat depots. Incubation of differentiated 3T3-L1 adipocytes with media of L. mesenteroides EH-1, a butyric acid-producing strain, significantly reduced the amounts of lipid droplets. The oral administration of L. mesenteroides EH-1 produced large amounts (>1 mM) of butyric acid in cecum and attenuated the HFD-induced upregulation of PPAR-γ and accumulation of abdominal fats in mice. The combination of 2% glucose with L. mesenteroides EH-1 increased the production of butyric acid and potentiated the probiotic activity of L. mesenteroides EH-1 against the formation of lipid droplets in 3T3-L1 adipocytes as well as abdominal fats in HFD-fed mice. The inhibition of free fatty acid receptor 2 (Ffar2) by its antagonist, GLPG-0974, markedly diminished the probiotic effects of L. mesenteroides EH-1 plus glucose on the suppression of HFD-induced PPAR-γ and abdominal fats. Besides demonstrating the probiotic value of L. mesenteroides EH-1, our results highlight the possible therapy targeting the butyric acid-activated Ffar2 pathway to reduce abdominal fats.
Original language | English |
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Article number | 1180 |
Pages (from-to) | 1-12 |
Number of pages | 12 |
Journal | Microorganisms |
Volume | 8 |
Issue number | 8 |
DOIs | |
State | Published - Aug 2020 |
Keywords
- Abdominal fats
- Butyric acid
- High-fat diet
- Leuconostoc mesenteroides
- PPAR-γ
Fingerprint
Dive into the research topics of 'Mouse abdominal fat depots reduced by butyric acid-producing leuconostoc mesenteroides'. Together they form a unique fingerprint.Projects
- 3 Finished
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Vaccines Targeting P. Acnes in the Skin Microbiome(2/3)
Huang, C.-M. (PI)
1/08/20 → 31/07/21
Project: Research
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Analogs of Short-Chain Fatty Acids Derived from Skin Microbiome as Antibiotic Adjuvants for Treatment of Acne Vulgaris( II )
Huang, C.-M. (PI)
1/11/19 → 31/10/20
Project: Research
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