Monitoring the polysaccharide quality of Agaricus blazei in submerged culture by examining molecular weight distribution and TNF-α release capability of macrophage cell line RAW 264.7

Chin Hang Shu; Bor Jiun Wen; Ko Jung Lin

doi:10.1023/B:BILE.0000007066.00221.b9

Monitoring the polysaccharide quality of Agaricus blazei in submerged culture by examining molecular weight distribution and TNF-α release capability of macrophage cell line RAW 264.7

Chin Hang Shu, Bor Jiun Wen, Ko Jung Lin

Department of Chemical and Materials Engineering

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A method for monitoring the biological activity of broth polysaccharides of Agaricus blazei (AB-BP) in a submerged culture is described. The TNF-α releasing capability of AB-BP on the macrophage cell line, RAW 264.7, correlated with the molecular weight of AB-BP. The quality of polysaccharide in a submerged culture of Agaricus blazei was indirectly monitored by analyzing the distribution of its molecular weight within 1 h. The harvest time of the maximum polysaccharide production did not coincide with that of the maximum biological characteristics in the batch culture.

Original language	English
Pages (from-to)	2061-2064
Number of pages	4
Journal	Biotechnology Letters
Volume	25
Issue number	24
DOIs	https://doi.org/10.1023/B:BILE.0000007066.00221.b9
State	Published - Dec 2003

Keywords

Agaricus blazei
Molecular weight
Polysaccharide
Quality control
Tumor necrosis factor-α

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10.1023/B:BILE.0000007066.00221.b9

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title = "Monitoring the polysaccharide quality of Agaricus blazei in submerged culture by examining molecular weight distribution and TNF-α release capability of macrophage cell line RAW 264.7",

abstract = "A method for monitoring the biological activity of broth polysaccharides of Agaricus blazei (AB-BP) in a submerged culture is described. The TNF-α releasing capability of AB-BP on the macrophage cell line, RAW 264.7, correlated with the molecular weight of AB-BP. The quality of polysaccharide in a submerged culture of Agaricus blazei was indirectly monitored by analyzing the distribution of its molecular weight within 1 h. The harvest time of the maximum polysaccharide production did not coincide with that of the maximum biological characteristics in the batch culture.",

keywords = "Agaricus blazei, Molecular weight, Polysaccharide, Quality control, Tumor necrosis factor-α",

author = "Shu, {Chin Hang} and Wen, {Bor Jiun} and Lin, {Ko Jung}",

note = "Funding Information: The authors would like to thank the Council of Agriculture of the Republic of China, Taiwan, for financially supporting this research under Contract No. COA 90 AG-3.1.3-FD-Z3.",

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AU - Shu, Chin Hang

AU - Wen, Bor Jiun

AU - Lin, Ko Jung

N1 - Funding Information: The authors would like to thank the Council of Agriculture of the Republic of China, Taiwan, for financially supporting this research under Contract No. COA 90 AG-3.1.3-FD-Z3.

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N2 - A method for monitoring the biological activity of broth polysaccharides of Agaricus blazei (AB-BP) in a submerged culture is described. The TNF-α releasing capability of AB-BP on the macrophage cell line, RAW 264.7, correlated with the molecular weight of AB-BP. The quality of polysaccharide in a submerged culture of Agaricus blazei was indirectly monitored by analyzing the distribution of its molecular weight within 1 h. The harvest time of the maximum polysaccharide production did not coincide with that of the maximum biological characteristics in the batch culture.

AB - A method for monitoring the biological activity of broth polysaccharides of Agaricus blazei (AB-BP) in a submerged culture is described. The TNF-α releasing capability of AB-BP on the macrophage cell line, RAW 264.7, correlated with the molecular weight of AB-BP. The quality of polysaccharide in a submerged culture of Agaricus blazei was indirectly monitored by analyzing the distribution of its molecular weight within 1 h. The harvest time of the maximum polysaccharide production did not coincide with that of the maximum biological characteristics in the batch culture.

KW - Agaricus blazei

KW - Molecular weight

KW - Polysaccharide

KW - Quality control

KW - Tumor necrosis factor-α

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Monitoring the polysaccharide quality of Agaricus blazei in submerged culture by examining molecular weight distribution and TNF-α release capability of macrophage cell line RAW 264.7

Abstract

Keywords

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