Monitoring DNA hybridization with a simply manufactured GMR biosensor

Ting Jou Ding; Jiann Hwa Lue; Ya Lun Tsai; Tsung Hsun Yang; Jenq Yang Chang; Wen Yih Chen

Monitoring DNA hybridization with a simply manufactured GMR biosensor

Ting Jou Ding, Jiann Hwa Lue, Ya Lun Tsai, Tsung Hsun Yang, Jenq Yang Chang, Wen Yih Chen

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

In this letter, a real-time approach to identify the various DNA molecules hybridized of the integrated micro-fluid with the simplest manufactured guided-mode resonance (GMR) sensor is presented. By monitoring the resonant peak wavelength shift of GMR biosensor, the DNA hybridization experiment can be recognized and clarified. The biosensor exhibited sensitivities about 2.14nm in peak wavelength shift for the detection of the hybridizing of both the capture DNA and the probe DNA. Furthermore, the stability and reliability of nucleic acid hybridization on the GMR sensor are examined by combining the sensor with the fluidic elements. The results reveal that the dynamics of DNA hybridization on the GMR sensor can obviously be monitored in real time.

Original language	English
Pages (from-to)	1015-1019
Number of pages	5
Journal	Life Science Journal
Volume	9
Issue number	2
State	Published - 25 Jun 2012

Keywords

Biosensor
Cost down
DNA hybridization
Guided-mode resonance
Real time monitoring
Reliability
Simply manufacture
Stability

Cite this

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title = "Monitoring DNA hybridization with a simply manufactured GMR biosensor",

abstract = "In this letter, a real-time approach to identify the various DNA molecules hybridized of the integrated micro-fluid with the simplest manufactured guided-mode resonance (GMR) sensor is presented. By monitoring the resonant peak wavelength shift of GMR biosensor, the DNA hybridization experiment can be recognized and clarified. The biosensor exhibited sensitivities about 2.14nm in peak wavelength shift for the detection of the hybridizing of both the capture DNA and the probe DNA. Furthermore, the stability and reliability of nucleic acid hybridization on the GMR sensor are examined by combining the sensor with the fluidic elements. The results reveal that the dynamics of DNA hybridization on the GMR sensor can obviously be monitored in real time.",

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AU - Ding, Ting Jou

AU - Lue, Jiann Hwa

AU - Tsai, Ya Lun

AU - Yang, Tsung Hsun

AU - Chang, Jenq Yang

AU - Chen, Wen Yih

PY - 2012/6/25

Y1 - 2012/6/25

N2 - In this letter, a real-time approach to identify the various DNA molecules hybridized of the integrated micro-fluid with the simplest manufactured guided-mode resonance (GMR) sensor is presented. By monitoring the resonant peak wavelength shift of GMR biosensor, the DNA hybridization experiment can be recognized and clarified. The biosensor exhibited sensitivities about 2.14nm in peak wavelength shift for the detection of the hybridizing of both the capture DNA and the probe DNA. Furthermore, the stability and reliability of nucleic acid hybridization on the GMR sensor are examined by combining the sensor with the fluidic elements. The results reveal that the dynamics of DNA hybridization on the GMR sensor can obviously be monitored in real time.

AB - In this letter, a real-time approach to identify the various DNA molecules hybridized of the integrated micro-fluid with the simplest manufactured guided-mode resonance (GMR) sensor is presented. By monitoring the resonant peak wavelength shift of GMR biosensor, the DNA hybridization experiment can be recognized and clarified. The biosensor exhibited sensitivities about 2.14nm in peak wavelength shift for the detection of the hybridizing of both the capture DNA and the probe DNA. Furthermore, the stability and reliability of nucleic acid hybridization on the GMR sensor are examined by combining the sensor with the fluidic elements. The results reveal that the dynamics of DNA hybridization on the GMR sensor can obviously be monitored in real time.

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KW - Cost down

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KW - Guided-mode resonance

KW - Real time monitoring

KW - Reliability

KW - Simply manufacture

KW - Stability

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Monitoring DNA hybridization with a simply manufactured GMR biosensor

Abstract

Keywords

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