Modulating the structure and function of an aminoacyl-trna synthetase cofactor by biotinylation

Chih Yao Chang, Chia Pei Chang, Shruti Chakraborty, Shao Win Wang, Yi Kuan Tseng, Chien Chia Wang

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Arc1p is a yeast-specific tRNA-binding protein that forms a ternary complex with glutamyl-tRNA synthetase (GluRSc ) and methionyl-tRNA synthetase (MetRS) in the cytoplasm to regulate their catalytic activities and subcellular distributions. Despite Arc1p not being involved in any known biotin-dependent reaction, it is a natural target of biotin modification. Results presented herein show that biotin modification had no obvious effect on the growth-supporting activity, subcellular distribution, tRNA binding, or interactions of Arc1p with GluRSc and MetRS. Nevertheless, biotinylation of Arc1p was temperature dependent; raising the growth temperature from 30 to 37 ° C drastically reduced its biotinylation level. As a result, Arc1p purified from a yeast culture that had been grown overnight at 37 ° C was essentially biotin free. Non-biotinylated Arc1p was more heat stable, more flexible in structure, and more effective than its biotinylated counterpart in promoting glutamylation activity of the otherwise inactive GluRSc at 37 ° C in vitro. Our study suggests that the structure and function of Arc1p can be modulated via biotinylation in response to temperature changes.

Original languageEnglish
Pages (from-to)17102-17111
Number of pages10
JournalJournal of Biological Chemistry
Volume291
Issue number33
DOIs
StatePublished - 12 Aug 2016

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