miR-596 Modulates Melanoma Growth by Regulating Cell Survival and Death

Szu Mam Liu, Chen Huan Lin, Jean Lu, In Yu Lin, Mu Shiun Tsai, Ming Hong Chen, Nianhan Ma

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Tumors grow because cancer cells lack the ability to balance cell survival and death signaling pathways. miR-596, a microRNA located at the 8p23.3 locus, has been shown by the TCGA-Assembler to be deleted in a significant number of melanoma samples. Here, we also validated the low levels of miR-596 in melanoma compared to tissue nevi, and Kaplan−Meier curve analysis revealed that low miR-596 expression was associated with worse overall survival. Moreover, we showed that miR-596 overexpression effectively inhibited MAPK/ERK signaling, cell proliferation, migration, and invasion and increased the cell apoptosis of melanoma cells. In addition, we found that miR-596 directly targets MEK1 and two apoptotic proteins, MCL1, and BCL2L1, in melanoma cells. Our findings indicated that miR-596 is an important miRNA that both negatively regulates the MAPK/ERK signaling pathway by targeting MEK1 and modulates the apoptosis pathway by targeting MCL1 and BCL2L1, suggesting that miR-596 could be a therapeutic candidate for treating melanoma, and a prognostic factor for melanoma patients.

Original languageEnglish
Pages (from-to)911-921
Number of pages11
JournalJournal of Investigative Dermatology
Volume138
Issue number4
DOIs
StatePublished - Apr 2018

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