Microcalorimetric studies of the interactions of lysozyme with immobilized Cu(II): Effects of pH value and salt concentration

Wen Yih Chen, Jiunn Fwu Lee, Ching Fa Wu, Heng Kwong Tsao

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

In this investigation, employing a highly sensitive microcalorimcter, we measure the influence of pH value and salt concentration on the heat of interaction between lysozyme and CS-IDA-Cu(II) gel. The direct enthalpy measurement of the interaction provides thermodynamic information regarding the binding behavior of lysozyme toward the immobilized metal ion. The binding enthalpy altered by adsorbed lysozyme at various pH values and salt concentrations are measured. The findings, along with the reported binding isotherm, are discussed herein.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalJournal of Colloid and Interface Science
Volume190
Issue number1
DOIs
StatePublished - 1 Jun 1997

Keywords

  • Adsorption
  • IMA
  • Immobilized metal ion affinity
  • Lysoszyme
  • Microcalorimetry

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