Microcalorimetric studies of the interactions of imidazole with immobilized Cu(II): Effects of pH value and salt concentration

Ching Fa Wu, Wen Yih Chen, Jiunn Fwu Lee

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

In this investigation, we measured the influence of pH value and salt concentration on the heat of interaction between imidazole and CS-IDA-Cu(II) gel with a highly sensitive microcalorimeter. The direct enthalpy measurement of the interaction provides thermodynamic information regarding the binding behavior of imidazole toward the immobilized metal ion. The changes in binding enthalpy with the adsorbed amount of imidazole measured at various pH values and salt concentrations are discussed and the binding isotherm is reported. The binding and thermodynamic data obtained in this study can provide information on the mechanism and process of imidazole and protein binding with immobilized metal ions.

Original languageEnglish
Pages (from-to)236-242
Number of pages7
JournalJournal of Colloid and Interface Science
Volume183
Issue number1
DOIs
StatePublished - 15 Oct 1996

Keywords

  • adsorption
  • IMA
  • imidazole
  • immobilized metal ion affinity
  • microcalorimetry

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