TY - JOUR
T1 - Micro-computed tomography detection of gold nanoparticle-labelled mesenchymal stem cells in the rat subretinal layer
AU - Mok, Pooi Ling
AU - Leow, Sue Ngein
AU - Koh, Avin Ee Hwan
AU - Mohd Nizam, Hairul Harun
AU - Ding, Suet Lee Shirley
AU - Luu, Chi
AU - Ruhaslizan, Raduan
AU - Wong, Hon Seng
AU - Halim, Wan Haslina Wan Abdul
AU - Ng, Min Hwei
AU - Idrus, Ruszymah Binti Hj
AU - Chowdhury, Shiplu Roy
AU - Bastion, Catherine Mae Lynn
AU - Subbiah, Suresh Kumar
AU - Higuchi, Akon
AU - Alarfaj, Abdullah A.
AU - Then, Kong Yong
N1 - Publisher Copyright:
© 2017 by the authors; licensee MDPI, Basel, Switzerland.
PY - 2017/2/8
Y1 - 2017/2/8
N2 - Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of these cells, as visualized by an area of hyperdensity from the coronal section images of the rat head. In addition, we confirmed the cellular uptake of the gold nanoparticles by the mesenchymal stem cells using transmission electron microscopy. As opposed to other methods, the current protocol provides a simple, less labour-intensive and more efficient labelling mechanism for real-time cell tracking. Finally, we discuss the potential manipulations of gold nanoparticles in stem cells for cell replacement and cancer therapy in ocular disorders or diseases.
AB - Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of these cells, as visualized by an area of hyperdensity from the coronal section images of the rat head. In addition, we confirmed the cellular uptake of the gold nanoparticles by the mesenchymal stem cells using transmission electron microscopy. As opposed to other methods, the current protocol provides a simple, less labour-intensive and more efficient labelling mechanism for real-time cell tracking. Finally, we discuss the potential manipulations of gold nanoparticles in stem cells for cell replacement and cancer therapy in ocular disorders or diseases.
KW - Gold nanoparticles
KW - Mesenchymal stem cells
KW - Micro-computed tomography
KW - Rat subretinal layer
KW - Stem cell tracking
KW - Transmission electron microscopy
UR - http://www.scopus.com/inward/record.url?scp=85012066763&partnerID=8YFLogxK
U2 - 10.3390/ijms18020345
DO - 10.3390/ijms18020345
M3 - 期刊論文
C2 - 28208719
AN - SCOPUS:85012066763
SN - 1661-6596
VL - 18
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 2
M1 - 345
ER -