Measurements of movement and diffusion coefficients of single cells on polymeric surface from image analysis

Akon Higuchi, Ho Ling-Yi, Li Ya Huang, Hui Chen, Yi Jen Chen, Qing Dong Ling

Research output: Contribution to journalArticlepeer-review


Time-lapse digital images were taken every 30 s of PC12 cells cultured on polystyrene dishes, collagen-coated dishes and poly(L-lysine)-coated dishes in high-serum medium, low-serum medium and neurite outgrowth factor (NGF)-containing medium to investigate their diffusion coefficients (i.e., self-diffusion coefficients), D and specific movement (i.e., specific frequent movement) using image analysis and Fast Fourier Transform (FFT) analysis. D for these cells was found to fluctuate as a function of time, D varying between 0 and 0.08 μm2/s. The trend observed upon examination of average D values was: D in high-serum medium ≤ D in low-serum medium ≤ D in NGF-containing medium. Image analysis and FFT analysis of single cells cultured on polymeric dishes in these three media did not have any specific frequency of cell movement between 0 and 0.0167 Hz. The high diffusion coefficient and high amplitude of power spectra of PC12 cells in high-serum medium might be attributed to the high energy necessary for their continual suppression of the mitogen-activated protein kinase (MAPK) cascade and for them to maintain their undifferentiated state.

Original languageEnglish
Pages (from-to)1545-1558
Number of pages14
JournalJournal of Biomaterials Science, Polymer Edition
Issue number12
StatePublished - 1 Aug 2010


  • collagen
  • diffusion
  • fast fourier transform
  • frequency
  • PC12 cells
  • poly(L-LYSINE)


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