Mangrove Helps: Sonneratia alba-Synthesized Silver Nanoparticles Magnify Guppy Fish Predation Against Aedes aegypti Young Instars and Down-Regulate the Expression of Envelope (E) Gene in Dengue Virus (Serotype DEN-2)

Kadarkarai Murugan, Devakumar Dinesh, Manickam Paulpandi, Jayapal Subramaniam, R. Rakesh, Pandiyan Amuthavalli, Chellasamy Panneerselvam, Udaiyan Suresh, Chitravel Vadivalagan, Mohamad Saleh Alsalhi, Sandhanasamy Devanesan, Hui Wei, Akon Higuchi, Marcello Nicoletti, Angelo Canale, Giovanni Benelli

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The control of dengue vectors with effective tools is crucial. Here, we fabricated silver nanoparticles (AgNP) using a cheap method relying to a mangrove extract (Sonneratia alba) as a reducing and stabilizing agent. AgNP were characterized by UV–vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction. LC50of S. alba extract against Aedes aegypti ranged from 192.03 ppm (larva I) to 353.36 ppm (pupa). LC50of AgNP ranged from 3.15 (I) to 13.61 ppm (pupa). Sub-lethal doses of AgNP magnified predation rates of guppy fishes, Poecilia reticulata, against Ae. aegypti and Chironomus kiiensis larvae. Mangrove-fabricated AgNP were evaluated for their antimicrobial potential against Bacillus subtilis, Klebsiella pneumoniae, and Salmonella typhi, using the agar disc diffusion and minimum inhibitory concentration protocol. Notably, S. alba-synthesized AgNP tested at doses ranging from 5 to 15 µg/mL down-regulated the expression of the envelope (E) gene and protein in dengue virus (serotype DEN-2), while only little cytotoxicity rates (i.e. <15%) were detected on Vero cells when AgNP were tested at 10 µg/mL. Overall, this study pointed out the potential of S. alba-synthesized AgNP to develop eco-friendly nanoformulations effective against dengue virus and its mosquito vectors.

Original languageEnglish
Pages (from-to)437-461
Number of pages25
JournalJournal of Cluster Science
Volume28
Issue number1
DOIs
StatePublished - 1 Jan 2017

Keywords

  • Arbovirus
  • Biosafety
  • Green-synthesis
  • Poecilia reticulata
  • Sonneratia alba

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