Lens-free holographic microscopy for complete blood analysis on a chip

Yi Chun Chen; Chia Sheng Kuo; Zhi Zhong Wang; Hsiang Yu Lei; Can Hua Yang; Chen Han Huang

doi:10.1117/12.2543132

Lens-free holographic microscopy for complete blood analysis on a chip

Yi Chun Chen, Chia Sheng Kuo, Zhi Zhong Wang, Hsiang Yu Lei, Can Hua Yang, Chen Han Huang

Department of Biomedical Sciences and Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

4 Scopus citations

Abstract

Complete Blood Count (CBC) is a routine blood test program that is often prescribed by physicians. It analyzes the values of various blood cells and can be used for detection of related diseases and infections. Flow cytometry has been one of the widely used technique to count, characterize and classify blood cells in the previous studies. However, the technique suffers from time-consuming procedure for analyzing the target cell and the bulky design of the detection platform. Hence, we propose a high-throughput lensless holographic microscopy for rapid blood analysis and imaging in large field-of-view (FOV) of 30 mm2. The holographic technique can simultaneously record the amplitude and phase of the diffraction pattern, which are different due to cell sizes and materials for further cell counting. Then the original cell images are digitally reconstructed by scalar diffraction theory and inverse Fourier transform to identify red blood cells, white blood cells and platelets. The lens-free holographic platform has compact size that makes it easy-to-handle. The lens-free design is able to analyze the blood samples on a chip by using the spatial coherence light (LED) emanating from a pinhole, and imaging on the CMOS sensor with a spatial resolution of 1.67 μm. The application of a CBC can diagnose anemia, infections, and other disorders. We believe that the lensless on-chip holographic platform will be a cost-effective tool for point-of-care cytometry.

Original language	English
Title of host publication	Optical Diagnostics and Sensing XX
Subtitle of host publication	Toward Point-of-Care Diagnostics
Editors	Gerard L. Cote
Publisher	SPIE
ISBN (Electronic)	9781510632578
DOIs	https://doi.org/10.1117/12.2543132
State	Published - 2020
Event	Optical Diagnostics and Sensing XX: Toward Point-of-Care Diagnostics 2020 - San Francisco, United States Duration: 3 Feb 2020 → …

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11247
ISSN (Print)	1605-7422

Conference

Conference	Optical Diagnostics and Sensing XX: Toward Point-of-Care Diagnostics 2020
Country/Territory	United States
City	San Francisco
Period	3/02/20 → …

Keywords

Diffraction
Holographic
Point-of-care
Reconstruction

Access to Document

10.1117/12.2543132

Cite this

Chen, Y. C., Kuo, C. S., Wang, Z. Z., Lei, H. Y., Yang, C. H., & Huang, C. H. (2020). Lens-free holographic microscopy for complete blood analysis on a chip. In G. L. Cote (Ed.), Optical Diagnostics and Sensing XX: Toward Point-of-Care Diagnostics [112470I] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11247). SPIE. https://doi.org/10.1117/12.2543132

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title = "Lens-free holographic microscopy for complete blood analysis on a chip",

abstract = "Complete Blood Count (CBC) is a routine blood test program that is often prescribed by physicians. It analyzes the values of various blood cells and can be used for detection of related diseases and infections. Flow cytometry has been one of the widely used technique to count, characterize and classify blood cells in the previous studies. However, the technique suffers from time-consuming procedure for analyzing the target cell and the bulky design of the detection platform. Hence, we propose a high-throughput lensless holographic microscopy for rapid blood analysis and imaging in large field-of-view (FOV) of 30 mm2. The holographic technique can simultaneously record the amplitude and phase of the diffraction pattern, which are different due to cell sizes and materials for further cell counting. Then the original cell images are digitally reconstructed by scalar diffraction theory and inverse Fourier transform to identify red blood cells, white blood cells and platelets. The lens-free holographic platform has compact size that makes it easy-to-handle. The lens-free design is able to analyze the blood samples on a chip by using the spatial coherence light (LED) emanating from a pinhole, and imaging on the CMOS sensor with a spatial resolution of 1.67 μm. The application of a CBC can diagnose anemia, infections, and other disorders. We believe that the lensless on-chip holographic platform will be a cost-effective tool for point-of-care cytometry.",

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author = "Chen, {Yi Chun} and Kuo, {Chia Sheng} and Wang, {Zhi Zhong} and Lei, {Hsiang Yu} and Yang, {Can Hua} and Huang, {Chen Han}",

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Chen, YC, Kuo, CS, Wang, ZZ, Lei, HY, Yang, CH & Huang, CH 2020, Lens-free holographic microscopy for complete blood analysis on a chip. in GL Cote (ed.), Optical Diagnostics and Sensing XX: Toward Point-of-Care Diagnostics., 112470I, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11247, SPIE, Optical Diagnostics and Sensing XX: Toward Point-of-Care Diagnostics 2020, San Francisco, United States, 3/02/20. https://doi.org/10.1117/12.2543132

Lens-free holographic microscopy for complete blood analysis on a chip. / Chen, Yi Chun; Kuo, Chia Sheng; Wang, Zhi Zhong et al.
Optical Diagnostics and Sensing XX: Toward Point-of-Care Diagnostics. ed. / Gerard L. Cote. SPIE, 2020. 112470I (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11247).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Lens-free holographic microscopy for complete blood analysis on a chip

AU - Chen, Yi Chun

AU - Kuo, Chia Sheng

AU - Wang, Zhi Zhong

AU - Lei, Hsiang Yu

AU - Yang, Can Hua

AU - Huang, Chen Han

PY - 2020

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N2 - Complete Blood Count (CBC) is a routine blood test program that is often prescribed by physicians. It analyzes the values of various blood cells and can be used for detection of related diseases and infections. Flow cytometry has been one of the widely used technique to count, characterize and classify blood cells in the previous studies. However, the technique suffers from time-consuming procedure for analyzing the target cell and the bulky design of the detection platform. Hence, we propose a high-throughput lensless holographic microscopy for rapid blood analysis and imaging in large field-of-view (FOV) of 30 mm2. The holographic technique can simultaneously record the amplitude and phase of the diffraction pattern, which are different due to cell sizes and materials for further cell counting. Then the original cell images are digitally reconstructed by scalar diffraction theory and inverse Fourier transform to identify red blood cells, white blood cells and platelets. The lens-free holographic platform has compact size that makes it easy-to-handle. The lens-free design is able to analyze the blood samples on a chip by using the spatial coherence light (LED) emanating from a pinhole, and imaging on the CMOS sensor with a spatial resolution of 1.67 μm. The application of a CBC can diagnose anemia, infections, and other disorders. We believe that the lensless on-chip holographic platform will be a cost-effective tool for point-of-care cytometry.

AB - Complete Blood Count (CBC) is a routine blood test program that is often prescribed by physicians. It analyzes the values of various blood cells and can be used for detection of related diseases and infections. Flow cytometry has been one of the widely used technique to count, characterize and classify blood cells in the previous studies. However, the technique suffers from time-consuming procedure for analyzing the target cell and the bulky design of the detection platform. Hence, we propose a high-throughput lensless holographic microscopy for rapid blood analysis and imaging in large field-of-view (FOV) of 30 mm2. The holographic technique can simultaneously record the amplitude and phase of the diffraction pattern, which are different due to cell sizes and materials for further cell counting. Then the original cell images are digitally reconstructed by scalar diffraction theory and inverse Fourier transform to identify red blood cells, white blood cells and platelets. The lens-free holographic platform has compact size that makes it easy-to-handle. The lens-free design is able to analyze the blood samples on a chip by using the spatial coherence light (LED) emanating from a pinhole, and imaging on the CMOS sensor with a spatial resolution of 1.67 μm. The application of a CBC can diagnose anemia, infections, and other disorders. We believe that the lensless on-chip holographic platform will be a cost-effective tool for point-of-care cytometry.

KW - Diffraction

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KW - Point-of-care

KW - Reconstruction

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M3 - 會議論文篇章

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ER -

Lens-free holographic microscopy for complete blood analysis on a chip

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Keywords

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