Lens-free holographic microscopy for complete blood analysis on a chip

Yi Chun Chen, Chia Sheng Kuo, Zhi Zhong Wang, Hsiang Yu Lei, Can Hua Yang, Chen Han Huang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

Complete Blood Count (CBC) is a routine blood test program that is often prescribed by physicians. It analyzes the values of various blood cells and can be used for detection of related diseases and infections. Flow cytometry has been one of the widely used technique to count, characterize and classify blood cells in the previous studies. However, the technique suffers from time-consuming procedure for analyzing the target cell and the bulky design of the detection platform. Hence, we propose a high-throughput lensless holographic microscopy for rapid blood analysis and imaging in large field-of-view (FOV) of 30 mm2. The holographic technique can simultaneously record the amplitude and phase of the diffraction pattern, which are different due to cell sizes and materials for further cell counting. Then the original cell images are digitally reconstructed by scalar diffraction theory and inverse Fourier transform to identify red blood cells, white blood cells and platelets. The lens-free holographic platform has compact size that makes it easy-to-handle. The lens-free design is able to analyze the blood samples on a chip by using the spatial coherence light (LED) emanating from a pinhole, and imaging on the CMOS sensor with a spatial resolution of 1.67 μm. The application of a CBC can diagnose anemia, infections, and other disorders. We believe that the lensless on-chip holographic platform will be a cost-effective tool for point-of-care cytometry.

Original languageEnglish
Title of host publicationOptical Diagnostics and Sensing XX
Subtitle of host publicationToward Point-of-Care Diagnostics
EditorsGerard L. Cote
PublisherSPIE
ISBN (Electronic)9781510632578
DOIs
StatePublished - 2020
EventOptical Diagnostics and Sensing XX: Toward Point-of-Care Diagnostics 2020 - San Francisco, United States
Duration: 3 Feb 2020 → …

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11247
ISSN (Print)1605-7422

Conference

ConferenceOptical Diagnostics and Sensing XX: Toward Point-of-Care Diagnostics 2020
Country/TerritoryUnited States
CitySan Francisco
Period3/02/20 → …

Keywords

  • Diffraction
  • Holographic
  • Point-of-care
  • Reconstruction

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