Least invasive in vivo imaging using harmonic generation microscopy

Szu Yu Chen, Shih Peng Tai, Cho Shuen Hsieh, Chao Yu Chen, Che Hang Yu, Yen Wei Lee, Wen Jeng Lee, Shee Uan Chen, Fu Hsiung Chang, Chi Kuang Sun

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations


In this manuscript, we review the physics and recent developments of the least invasive optical higher harmonic generation microscopy, with an emphasis on the in vivo molecular imaging applications. Optical higher harmonicgenerations, including second harmonic generation (SHG) and third harmonic generation (THG), leave no energy deposition to the interacted matters due to their energy-conservation characteristic, providing the "noninvasiveness" nature desirable for clinical studies. Combined with their nonlinearity, harmonic generation microscopy provides threedimensional sectioning capability, offering new insights into live samples. By choosing the lasers working in the high penetration window, we have recently developed a least-invasive in vivo light microscopy with submicron 3D resolution and high penetration, utilizing endogenous and resonantly-enhanced multi-harmonic- generation signals in live specimens, with focused applications on the developmental biology study and clinical virtual biopsy.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences VIII
StatePublished - 2008
EventMultiphoton Microscopy in the Biomedical Sciences VIII - San Jose, CA, United States
Duration: 20 Jan 200822 Jan 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceMultiphoton Microscopy in the Biomedical Sciences VIII
Country/TerritoryUnited States
CitySan Jose, CA


  • Harmonic generation microscopy
  • Higher harmonic generation
  • Molecular imaging
  • Noninvasive microscopy
  • Resonance enhancement
  • Second harmonic generation
  • Third harmonic generation
  • Virtual biopsy


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