Label-free detection of prostate-specific antigen with FBAR-based sensor with oriented antibody immobilization

Anderson Lin, Yi Jia Li, Lingtao Wang, Shih Jui Chen, Mitchell E. Gross, Eun Sok Kim

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

7 Scopus citations

Abstract

This paper reports a real-time, label-free detection of prostate-specific-antigen (PSA) through mass sensing with film bulk acoustic resonator (FBAR) coated with an oriented antibody layer. The oriented antibody layer is produced with protein A forming a polarized bond between antibody, at the Fc (fragment crystallizable) region of an IgG (immunoglobulin G), and a gold layer. The first prototype sensor is capable of detecting PSA concentration in hundreds of nanogram per milliliter range, and can be regenerated with 0.1 M glycine pH 2.5 that strips off only the antibody-antigen complex. Also, preliminary testing shows the absence of cross-reaction with bovine serum albumin (BSA). An improved version of the sensor utilizing DTSSP (3,3′-Dithiobis[sulfosuccinimidylpropionate]) crosslinker shows a better response, and increases the sensitivity to a level where PSA detection down to nanograms per milliliter range is possible.

Original languageEnglish
Title of host publication2011 IEEE International Ultrasonics Symposium, IUS 2011
Pages797-800
Number of pages4
DOIs
StatePublished - 2011
Event2011 IEEE International Ultrasonics Symposium, IUS 2011 - Orlando, FL, United States
Duration: 18 Oct 201121 Oct 2011

Publication series

NameIEEE International Ultrasonics Symposium, IUS
ISSN (Print)1948-5719
ISSN (Electronic)1948-5727

Conference

Conference2011 IEEE International Ultrasonics Symposium, IUS 2011
Country/TerritoryUnited States
CityOrlando, FL
Period18/10/1121/10/11

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