Kartogenin enhances chondrogenic differentiation of mscs in 3d tri-copolymer scaffolds and the self-designed bioreactor system

Ching Yun Chen, Chunching Li, Cherng Jyh Ke, Jui Sheng Sun, Feng Huei Lin

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Human cartilage has relatively slow metabolism compared to other normal tissues. Cartilage damage is of great clinical consequence since cartilage has limited intrinsic healing potential. Cartilage tissue engineering is a rapidly emerging field that holds great promise for tissue function repair and artificial/engineered tissue substitutes. However, current clinical therapies for cartilage repair are less than satisfactory and rarely recover full function or return the diseased tissue to its native healthy state. Kartogenin (KGN), a small molecule, can promote chondrocyte differentiation both in vitro and in vivo. The purpose of this research is to optimize the chondrogenic process in mesenchymal stem cell (MSC)-based chondrogenic constructs with KGN for potential use in cartilage tissue engineering. In this study, we demonstrate that KGN treatment can promote MSC condensation and cell cluster formation within a tri-copolymer scaffold. Expression of Acan, Sox9, and Col2a1 was significantly up-regulated in three-dimensional (3D) culture conditions. The lacuna-like structure showed active deposition of type II collagen and aggrecan deposition. We expect these results will open new avenues for the use of small molecules in chondrogenic differentiation protocols in combination with scaffolds, which may yield better strategies for cartilage tissue engineering.

Original languageEnglish
Article number115
Pages (from-to)1-20
Number of pages20
JournalBiomolecules
Volume11
Issue number1
DOIs
StatePublished - Jan 2021

Keywords

  • 3D culture
  • Chondrogenic differentiation
  • Kartogenin
  • MSCs
  • Self-designed bioreactor system
  • Tri-copolymer scaffolds

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