Investigating the effects of sodium dodecyl sulfate on the aggregative behavior of hen egg-white lysozyme at acidic pH

Ying Tz Hung, Ming Shen Lin, Wen Yih Chen, Steven Sheng Shih Wang

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The research presented here is aimed at examining the effects of sodium dodecyl sulfate on the aggregative behavior of hen egg-white lysozyme at pH 2.0. Through various spectroscopic techniques, dynamic light scattering, and electron microscopy, we first demonstrated that SDS exhibited a biphasic effect on lysozyme fibrillation. The presence of SDS at higher concentrations (e.g., 0.25, 5.00, or 20.00. mM SDS) was found to suppress fibril formation of lysozyme whereas fibrillogenic lysozyme-SDS ensemble containing β-sheet-rich conformation was observed upon the addition of lower concentrations of SDS (e.g., 0.00, 0.06, or 0.1. mM SDS). Next, our equilibrium urea-unfolding data revealed that lysozyme samples with higher SDS concentrations showed superior thermodynamic stabilities over the ones with no or lower levels of SDS. Finally, the correlation between SDS concentration and lysozyme aggregative/fibrillogenic propensity and the underlying interacting mechanism were further explored using surface tensiometry and isothermal titration calorimetry. We believe the outcome from this work may not only help decipher the molecular mechanism of amyloid fibrillation, but also shed light on a rational design of potential therapeutic strategies for amyloid pathology.

Original languageEnglish
Pages (from-to)141-151
Number of pages11
JournalColloids and Surfaces B: Biointerfaces
Volume81
Issue number1
DOIs
StatePublished - Nov 2010

Keywords

  • Aggregation
  • Amyloid fibril
  • Fibrillation
  • Hen lysozyme
  • Sodium dodecyl sulfate

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