Abstract
The cornea functions as an optical lens and plays an important role in vision. For corneal diagnosis and treatment such as refractive surgery, a microscopic imaging system with a 3-D cellular resolution and retinal safety is strongly desired. Recently, confocal and multiphoton microscopies have been applied to corneal imaging with visible to near-infrared light sources. To increase retinal safety, an infrared light source is be needed. In this work, an infrared-based third and second harmonic generation microscopic study of mouse eyes is reported with ∼700-μm penetrability and high cellular resolution. This study provides a critical reference for future development of infrared-based corneal imaging.
| Original language | English |
|---|---|
| Article number | 044012 |
| Journal | Journal of Biomedical Optics |
| Volume | 14 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2009 |
Keywords
- cornea
- high penetrability
- high resolution
- infrared based
- mouse eye
- second harmonic generation
- third harmonic generation