Infrared-based third and second harmonic generation imaging of cornea

Szu Yu Chen, Han Chieh Yu, I. Jong Wang, Chi Kuang Sun

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


The cornea functions as an optical lens and plays an important role in vision. For corneal diagnosis and treatment such as refractive surgery, a microscopic imaging system with a 3-D cellular resolution and retinal safety is strongly desired. Recently, confocal and multiphoton microscopies have been applied to corneal imaging with visible to near-infrared light sources. To increase retinal safety, an infrared light source is be needed. In this work, an infrared-based third and second harmonic generation microscopic study of mouse eyes is reported with ∼700-μm penetrability and high cellular resolution. This study provides a critical reference for future development of infrared-based corneal imaging.

Original languageEnglish
Article number044012
JournalJournal of Biomedical Optics
Issue number4
StatePublished - 2009


  • cornea
  • high penetrability
  • high resolution
  • infrared based
  • mouse eye
  • second harmonic generation
  • third harmonic generation


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