Induction of apoptosis by high-dose gold nanoparticles in nasopharyngeal carcinoma cells

Ming Ying Lan, Yen Bin Hsu, Chih Hung Hsu, Ching Yin Ho, Jin Ching Lin, Sheng Wei Lee

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Objective: Nasopharyngeal carcinoma (NPC) is a rare malignancy in most parts of the world, but is a common cancer in southern Asia. Local recurrent disease and distant metastasis of NPC are still the unsolved problems. Recently, gold nanoparticles (AuNPs) have been developed as potential in vivo diagnostic and therapeutic agents. However, their role on nasopharyngeal cancer remains unknown. The object of this study is to investigate if AuNPs can be used as a new therapeutic agent for NPC by evaluating their anti-tumor effect in vitro. Methods: The AuNPs were prepared by the reduction of chloroauric acid to neutral gold. Their size distribution and microstructures were characterized by transmission electron microscopy (TEM). To evaluate their cytotoxic effect, NPC cell line TW01 and Human Nasal Epithelial Cells (HNEpC) were cultured in various concentrations of AuNPs for 3 days. Cell viability was evaluated by Trypan Blue viability assay while morphologic findings were observed via light microscopy. Terminal deoxynucleotidyltransferase-mediated dUPT nick end labeling (TUNEL) assay was used to detect apoptosis. Results: AuNPs prepared in this study had an average diameter of 20.5. nm and they were observed under light microscopy as dark material aggregated in the cells after treatment. Contrary to the HNEpC, the AuNPs reduced cell viability of NPC cell in a concentration-dependant manner by Trypan Blue assay, especially at high concentration. Besides, cell apoptosis was demonstrated by positive TUNEL assay. Conclusions: The AuNP possesses specific imaging properties and is cytotoxic to NPC cells at high concentrations.

Original languageEnglish
Pages (from-to)563-568
Number of pages6
JournalAuris Nasus Larynx
Volume40
Issue number6
DOIs
StatePublished - Dec 2013

Keywords

  • Anti-tumor
  • Cancer
  • Cytotoxicity
  • Gold nanoparticle
  • Nasopharyngeal carcinoma

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