In vivo harmonic generation biopsy of human skin

Szu Yu Chen, Hai Yin Wu, Chi Kuang Sun

Research output: Contribution to journalArticlepeer-review

81 Scopus citations


The ability to in vivo image deep tissues noninvasively with a high resolution is strongly required for optical virtual biopsy. Higher harmonic generation microscopy, combined with second- and third-harmonic generation microscopies, is applied to 17 Asian volunteers' forearm skin. After continuous observation for 30 min, no visible damage was found. Our study proves that harmonic generation biopsy (HGB) is able to satisfy the safety requirement and to provide high penetrability (∼300 μm) and submicron resolution all at the same time and is a promising tool for future virtual biopsy of skin diseases. In contrast to a previous study on fixed human skin specimens, a much improved penetrability and much reduced resolution-degradation versus depth are found in this in vivo examination.

Original languageEnglish
Article number060505
JournalJournal of Biomedical Optics
Issue number6
StatePublished - 2009


  • Dermatology
  • Harmonic generation microscopy
  • High penetrability
  • High resolution
  • Human skin
  • In vivo
  • Noninvasive


Dive into the research topics of 'In vivo harmonic generation biopsy of human skin'. Together they form a unique fingerprint.

Cite this