TY - JOUR
T1 - In vitro evaluation of injectable Tideglusib-loaded hyaluronic acid hydrogels incorporated with Rg1-loaded chitosan microspheres for vital pulp regeneration
AU - Atila, Deniz
AU - Chen, Ching Yun
AU - Lin, Chun Pin
AU - Lee, Yuan Ling
AU - Hasirci, Vasif
AU - Tezcaner, Ayşen
AU - Lin, Feng Huei
N1 - Publisher Copyright:
© 2021
PY - 2022/2/15
Y1 - 2022/2/15
N2 - Injectable systems receive attention in endodontics due to the complicated and irregular anatomical structure of root canals. Here, injectable Tideglusib (Td)-loaded hyaluronic acid hydrogels (HAH) incorporated with Rg1-loaded chitosan microspheres (CSM) were developed for vital pulp regeneration, providing release of Td and Rg1 to trigger odontoblastic differentiation of human dental pulp stem cells (DPSC) by Td and vascularization of pulp by Rg1. The optimal concentrations were determined as 90 nM and 50 μg/mL for Td and Rg1, and loaded in HA and CSM in HAH, respectively. Odontogenic (COL1A1, ALP, OCN, Axin-2, DSPP, and DMP1) and angiogenic (VEGFA, VEGFR2, and eNOS) differentiation of DPSC cultured in the presence of hydrogels was shown at gene expression level. Our results suggest that our injectable hydrogel formulation has potential to improve strategies for vital pulp regeneration. In vivo evaluations are needed to test the feasibility and potential of these hydrogels for vital pulp regeneration.
AB - Injectable systems receive attention in endodontics due to the complicated and irregular anatomical structure of root canals. Here, injectable Tideglusib (Td)-loaded hyaluronic acid hydrogels (HAH) incorporated with Rg1-loaded chitosan microspheres (CSM) were developed for vital pulp regeneration, providing release of Td and Rg1 to trigger odontoblastic differentiation of human dental pulp stem cells (DPSC) by Td and vascularization of pulp by Rg1. The optimal concentrations were determined as 90 nM and 50 μg/mL for Td and Rg1, and loaded in HA and CSM in HAH, respectively. Odontogenic (COL1A1, ALP, OCN, Axin-2, DSPP, and DMP1) and angiogenic (VEGFA, VEGFR2, and eNOS) differentiation of DPSC cultured in the presence of hydrogels was shown at gene expression level. Our results suggest that our injectable hydrogel formulation has potential to improve strategies for vital pulp regeneration. In vivo evaluations are needed to test the feasibility and potential of these hydrogels for vital pulp regeneration.
KW - Chitosan microspheres
KW - Hyaluronic acid
KW - Injectable hydrogels
KW - Rg1
KW - Tideglusib
KW - Vital pulp regeneration
UR - http://www.scopus.com/inward/record.url?scp=85120942689&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2021.118976
DO - 10.1016/j.carbpol.2021.118976
M3 - 期刊論文
C2 - 34973790
AN - SCOPUS:85120942689
SN - 0144-8617
VL - 278
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 118976
ER -