In vitro evaluation of injectable Tideglusib-loaded hyaluronic acid hydrogels incorporated with Rg1-loaded chitosan microspheres for vital pulp regeneration

Deniz Atila, Ching Yun Chen, Chun Pin Lin, Yuan Ling Lee, Vasif Hasirci, Ayşen Tezcaner, Feng Huei Lin

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Injectable systems receive attention in endodontics due to the complicated and irregular anatomical structure of root canals. Here, injectable Tideglusib (Td)-loaded hyaluronic acid hydrogels (HAH) incorporated with Rg1-loaded chitosan microspheres (CSM) were developed for vital pulp regeneration, providing release of Td and Rg1 to trigger odontoblastic differentiation of human dental pulp stem cells (DPSC) by Td and vascularization of pulp by Rg1. The optimal concentrations were determined as 90 nM and 50 μg/mL for Td and Rg1, and loaded in HA and CSM in HAH, respectively. Odontogenic (COL1A1, ALP, OCN, Axin-2, DSPP, and DMP1) and angiogenic (VEGFA, VEGFR2, and eNOS) differentiation of DPSC cultured in the presence of hydrogels was shown at gene expression level. Our results suggest that our injectable hydrogel formulation has potential to improve strategies for vital pulp regeneration. In vivo evaluations are needed to test the feasibility and potential of these hydrogels for vital pulp regeneration.

Original languageEnglish
Article number118976
JournalCarbohydrate Polymers
Volume278
DOIs
StatePublished - 15 Feb 2022

Keywords

  • Chitosan microspheres
  • Hyaluronic acid
  • Injectable hydrogels
  • Rg1
  • Tideglusib
  • Vital pulp regeneration

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