In-situ formation of “electron conductive wires” threaded ZIF-8 membrane for multiplexed immunoassay of human interleukins

Yu Lv, Mingshi Deng, Xuefeng Wang, Xinghua Gao, Lingyan Feng, Wei Chen, Chung Chiun Liu, Xinxin Li, Yuan Zhang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


To address the challenge of highly sensitive and accurate detection of biomarkers in complex environments, a rational engineering strategy for designing electrochemical immunosensing platform is proposed. Herein, we develop a microsensor chip through the combination of multiplexed electrodes and microfluidic channels for the parallel detection of human interleukins (IL-6 and IL-8). For the construction of an efficient sensing interface, the conductive silver nanowires (Ag NWs) wrapped with zeolitic imidazolate framework (ZIF-8) thin film (denoted as ZIF-8@Ag NWs) are prepared, and then employed for the multi-functionalization of electrodes. The immunodetection of ILs is based on the direct signal transduction ability of Ag NWs and specific interaction of periodically arranged Zn2+ ions in ZIF-8 films with biomolecules, which offer the high assay sensitivity and good specificity. The immunosensor chip achieves a wide detection range from pg/mL to ng/mL and possesses the ability to resist non-specific proteins adsorption in biological complex media. Further clinical serum samples assay verifies that the combination of IL-6 and IL-8 levels yields high diagnostic accuracy. Principal component analysis (PCA) reveals that 18 patient samples could be fully separated from healthy control samples. The low-cost, easily fabricated electrochemical immunosensing platform provides a rapid serum test for diagnosis and personalized therapy of inflammatory diseases, and can also be generalized to other immunoreaction-based biomarkers detections. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)2866-2874
Number of pages9
JournalNano Research
Issue number2
StatePublished - Feb 2023


  • biomarker detection
  • integrated nanostructure
  • interleukins
  • microsensor
  • zeolitic imidazolate framework (ZIF)


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