Improving resolution of second harmonic generation microscopy via scanning structured illumination

Chia Hua Yeh, Cheng Zn Tan, Ching Hsiao Arthur Cheng, Jui Ting Hung, Szu Yu Chen

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Second harmonic generation microscopy (SHGM) is a well-known technique for examining the noncentrosymmetric structures in biomedical research. However, without real-state transitions, fluorescence-based superresolution methods cannot be applied. To improve the resolution, fringe-scanning SHGM (FS-SHGM), which combines SHGM with structured illumination based on point-scanning, is introduced in this paper. The scanning path was modulated to generate illumination patterns. For the coherent parts of SHG signals, a mathematical model of image formation and reconstruction was established. Both simulations and experiments showed a resolution improvement factor of ~1.4 in the lateral and 1.56 in the axial directions for chicken tendons and mouse skin.

Original languageEnglish
Article number#342072
Pages (from-to)6081-6090
Number of pages10
JournalBiomedical Optics Express
Volume9
Issue number12
DOIs
StatePublished - 1 Dec 2018

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