TY - JOUR
T1 - Improving resolution of second harmonic generation microscopy via scanning structured illumination
AU - Yeh, Chia Hua
AU - Tan, Cheng Zn
AU - Cheng, Ching Hsiao Arthur
AU - Hung, Jui Ting
AU - Chen, Szu Yu
N1 - Publisher Copyright:
© 2018 Optical Society of America.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Second harmonic generation microscopy (SHGM) is a well-known technique for examining the noncentrosymmetric structures in biomedical research. However, without real-state transitions, fluorescence-based superresolution methods cannot be applied. To improve the resolution, fringe-scanning SHGM (FS-SHGM), which combines SHGM with structured illumination based on point-scanning, is introduced in this paper. The scanning path was modulated to generate illumination patterns. For the coherent parts of SHG signals, a mathematical model of image formation and reconstruction was established. Both simulations and experiments showed a resolution improvement factor of ~1.4 in the lateral and 1.56 in the axial directions for chicken tendons and mouse skin.
AB - Second harmonic generation microscopy (SHGM) is a well-known technique for examining the noncentrosymmetric structures in biomedical research. However, without real-state transitions, fluorescence-based superresolution methods cannot be applied. To improve the resolution, fringe-scanning SHGM (FS-SHGM), which combines SHGM with structured illumination based on point-scanning, is introduced in this paper. The scanning path was modulated to generate illumination patterns. For the coherent parts of SHG signals, a mathematical model of image formation and reconstruction was established. Both simulations and experiments showed a resolution improvement factor of ~1.4 in the lateral and 1.56 in the axial directions for chicken tendons and mouse skin.
UR - http://www.scopus.com/inward/record.url?scp=85057888737&partnerID=8YFLogxK
U2 - 10.1364/BOE.9.006081
DO - 10.1364/BOE.9.006081
M3 - 期刊論文
AN - SCOPUS:85057888737
SN - 2156-7085
VL - 9
SP - 6081
EP - 6090
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 12
M1 - #342072
ER -