Improving pharmaceutical protein production in Oryza sativa

Yu Chieh Kuo, Chia Chun Tan, Jung Ting Ku, Wei Cho Hsu, Sung Chieh Su, Chung An Lu, Li Fen Huang

Research output: Contribution to journalReview articlepeer-review

34 Scopus citations

Abstract

Application of plant expression systems in the production of recombinant proteins has several advantages, such as low maintenance cost, absence of human pathogens, and possession of complex post-translational glycosylation capabilities. Plants have been successfully used to produce recombinant cytokines, vaccines, antibodies, and other proteins, and rice (Oryza sativa) is a potential plant used as recombinant protein expression system. After successful transformation, transgenic rice cells can be either regenerated into whole plants or grown as cell cultures that can be upscaled into bioreactors. This review summarizes recent advances in the production of different recombinant protein produced in rice and describes their production methods as well as methods to improve protein yield and quality. Glycosylation and its impact in plant development and protein production are discussed, and several methods of improving yield and quality that have not been incorporated in rice expression systems are also proposed. Finally, different bioreactor options are explored and their advantages are analyzed.

Original languageEnglish
Pages (from-to)8719-8739
Number of pages21
JournalInternational Journal of Molecular Sciences
Volume14
Issue number5
DOIs
StatePublished - May 2013

Keywords

  • Bioreactor
  • Glycosylation
  • Inducible system
  • Oryza sativa
  • Recombinant protein
  • Suspension cells
  • Transgenic plants

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