Hollow fiber bioreactor for high density and continuous production of carcinoembryonic antigen

Akon Higuchi; Hirokazu Takarada; Jun Uchino; Yoshitaka Tanigaki; Mariko Hara

doi:10.2115/fiber.54.4_204

Hollow fiber bioreactor for high density and continuous production of carcinoembryonic antigen

Akon Higuchi, Hirokazu Takarada, Jun Uchino, Yoshitaka Tanigaki, Mariko Hara

Department of Chemical and Materials Engineering

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Continuous cultivation of CW2 cells (a human colorectal adenocarcinoma tumor cell) using a hollow fiber bioreactor was investigated for the production of carcinoembryonic antigen (CEA) over 34 days. The CEA production rate increased with the increase in incubation time up to 20 days and was found to be approximately a constant value, 20 ng/cm² day, after the incubation time of 20 days. The CEA production rate was known to vary in different cell lines. The CEA production rates were compared with those obtained in this study and in the literature using hollow fiber bioreactors. It was found that CW2 cells produced CEA moderately among those cell lines. The high cell density (9.1 × 10⁶ cells/cm²) achieved in the hollow fiber bioreactor compared to the flask culture (4.0 × 10⁵ cells/cm²) made it possible to obtain continuous high production of CEA over an extended time period.

Original language	English
Pages (from-to)	204-211
Number of pages	8
Journal	Sen'i Gakkaishi
Volume	54
Issue number	4
DOIs	https://doi.org/10.2115/fiber.54.4_204
State	Published - Jan 1998

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title = "Hollow fiber bioreactor for high density and continuous production of carcinoembryonic antigen",

abstract = "Continuous cultivation of CW2 cells (a human colorectal adenocarcinoma tumor cell) using a hollow fiber bioreactor was investigated for the production of carcinoembryonic antigen (CEA) over 34 days. The CEA production rate increased with the increase in incubation time up to 20 days and was found to be approximately a constant value, 20 ng/cm2 day, after the incubation time of 20 days. The CEA production rate was known to vary in different cell lines. The CEA production rates were compared with those obtained in this study and in the literature using hollow fiber bioreactors. It was found that CW2 cells produced CEA moderately among those cell lines. The high cell density (9.1 × 106 cells/cm2) achieved in the hollow fiber bioreactor compared to the flask culture (4.0 × 105 cells/cm2) made it possible to obtain continuous high production of CEA over an extended time period.",

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T1 - Hollow fiber bioreactor for high density and continuous production of carcinoembryonic antigen

AU - Higuchi, Akon

AU - Takarada, Hirokazu

AU - Uchino, Jun

AU - Tanigaki, Yoshitaka

AU - Hara, Mariko

PY - 1998/1

Y1 - 1998/1

N2 - Continuous cultivation of CW2 cells (a human colorectal adenocarcinoma tumor cell) using a hollow fiber bioreactor was investigated for the production of carcinoembryonic antigen (CEA) over 34 days. The CEA production rate increased with the increase in incubation time up to 20 days and was found to be approximately a constant value, 20 ng/cm2 day, after the incubation time of 20 days. The CEA production rate was known to vary in different cell lines. The CEA production rates were compared with those obtained in this study and in the literature using hollow fiber bioreactors. It was found that CW2 cells produced CEA moderately among those cell lines. The high cell density (9.1 × 106 cells/cm2) achieved in the hollow fiber bioreactor compared to the flask culture (4.0 × 105 cells/cm2) made it possible to obtain continuous high production of CEA over an extended time period.

AB - Continuous cultivation of CW2 cells (a human colorectal adenocarcinoma tumor cell) using a hollow fiber bioreactor was investigated for the production of carcinoembryonic antigen (CEA) over 34 days. The CEA production rate increased with the increase in incubation time up to 20 days and was found to be approximately a constant value, 20 ng/cm2 day, after the incubation time of 20 days. The CEA production rate was known to vary in different cell lines. The CEA production rates were compared with those obtained in this study and in the literature using hollow fiber bioreactors. It was found that CW2 cells produced CEA moderately among those cell lines. The high cell density (9.1 × 106 cells/cm2) achieved in the hollow fiber bioreactor compared to the flask culture (4.0 × 105 cells/cm2) made it possible to obtain continuous high production of CEA over an extended time period.

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JO - Sen'i Gakkaishi

JF - Sen'i Gakkaishi

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Hollow fiber bioreactor for high density and continuous production of carcinoembryonic antigen

Abstract

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