Hemocompatible mixed-charge copolymer brushes of pseudozwitterionic surfaces resistant to nonspecific plasma protein fouling

Yung Chang, Shih Hung Shu, Yu Ju Shih, Chih Wei Chu, Ruoh Chyu Ruaan, Wen Yih Chen

Research output: Contribution to journalArticlepeer-review

129 Scopus citations

Abstract

In this work, the hemocompatibility of a sulfobetaine-like copolymer brush resulting from a mixed-charge copolymerization of the positively charged 11 -mercapto-N,N,N-trimethylammonium chloride (TMA) and negatively charged 11 -mercaptoundecylsulfonic acid (SA) was studied. Mixed charge distribution in the prepared. poly(TMA-co-SA) copolymer brushes was controlled by the regulation of the reaction rate of the surface-initiated atom transfer radical polymerization (ATRP). The adsorption behavior of plasma proteins on a surface grafted with poly(TMA-co-SA) was measured by a surface plasmon resonance (SPR) sensor. The effects of varying temperature, solution pH, and ionic strength on the antifouling characteristics of the mixed-charge copolymer brushes were systematically evaluated, and the protein-fouling resistance was discussed, in detail, especially with respect to the effect of ionic strength on the intra- and intermolecular interactions of the poly(TMA-co-SA) with proteins. The adhesion and activation of blood cells on the poly(TMA-co-SA)-grafted surface in contact with human whole blood was also demonstrated. The results suggest that mixed-charge copolymer brushes of poly(TMA-co-SA), which, like zwitterionic homopolymer brushes, have overall charge neutrality, can be used, in similar applications for protein-fouling resistance and have excellent hemocompatibility with human whole blood at physiologic temperatures.

Original languageEnglish
Pages (from-to)3522-3530
Number of pages9
JournalLangmuir
Volume26
Issue number5
DOIs
StatePublished - 2 Mar 2010

Fingerprint

Dive into the research topics of 'Hemocompatible mixed-charge copolymer brushes of pseudozwitterionic surfaces resistant to nonspecific plasma protein fouling'. Together they form a unique fingerprint.

Cite this