Green-synthesized silver nanoparticles as a novel control tool against dengue virus (DEN-2) and its primary vector Aedes aegypti

Vasu Sujitha, Kadarkarai Murugan, Manickam Paulpandi, Chellasamy Panneerselvam, Udaiyan Suresh, Mathath Roni, Marcello Nicoletti, Akon Higuchi, Pari Madhiyazhagan, Jayapal Subramaniam, Devakumar Dinesh, Chithravel Vadivalagan, Balamurugan Chandramohan, Abdullah A. Alarfaj, Murugan A. Munusamy, Donald R. Barnard, Giovanni Benelli

Research output: Contribution to journalArticlepeer-review

121 Scopus citations


Dengue is an arthropod-borne viral infection mainly vectored through the bite of Aedes mosquitoes. Recently, its transmission has strongly increased in urban and semi-urban areas of tropical and sub-tropical regions worldwide, becoming a major international public health concern. There is no specific treatment for dengue. Its prevention and control solely depends on effective vector control measures. In this study, we proposed the green-synthesis of silver nanoparticles (AgNP) as a novel and effective tool against the dengue serotype DEN-2 and its major vector Aedes aegypti. AgNP were synthesized using the Moringa oleifera seed extract as reducing and stabilizing agent. AgNP were characterized using a variety of biophysical methods including UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and sorted for size categories. AgNP showed in vitro antiviral activity against DEN-2 infecting vero cells. Viral titer was 7 log10 TCID50/ml in control (AgNP-free), while it dropped to 3.2 log10 TCID50/ml after a single treatment with 20 μl/ml of AgNP. After 6 h, DEN-2 yield was 5.8 log10 PFU/ml in the control, while it was 1.4 log10 PFU/ml post-treatment with AgNP (20 μl/ml). AgNP were highly effective against the dengue vector A. aegypti, with LC50 values ranging from 10.24 ppm (I instar larvae) to 21.17 ppm (pupae). Overall, this research highlighted the concrete potential of green-synthesized AgNP in the fight against dengue and its primary vector A. aegypti. Further research on structure–activity relationships of AgNP against other dengue serotypes is urgently required.

Original languageEnglish
Pages (from-to)3315-3325
Number of pages11
JournalParasitology Research
Issue number9
StatePublished - 18 Sep 2015


  • Aedes aegypti
  • Botanical insecticides
  • cytotoxicity
  • Moringa oleifera
  • Mosquito-borne diseases
  • silver nanoparticles


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