TY - JOUR
T1 - Generation of pluripotent stem cells without the use of genetic material
AU - Higuchi, Akon
AU - Ling, Qing Dong
AU - Kumar, S. Suresh
AU - Munusamy, Murugan A.
AU - Alarfaj, Abdullah A.
AU - Chang, Yung
AU - Kao, Shih Hsuan
AU - Lin, Ke Chen
AU - Wang, Han Chow
AU - Umezawa, Akihiro
N1 - Publisher Copyright:
© 2015 USCAP, Inc All rights reserved.
PY - 2015/1/31
Y1 - 2015/1/31
N2 - Induced pluripotent stem cells (iPSCs) provide a platform to obtain patient-specific cells for use as a cell source in regenerative medicine. Although iPSCs do not have the ethical concerns of embryonic stem cells, iPSCs have not been widely used in clinical applications, as they are generated by gene transduction. Recently, iPSCs have been generated without the use of genetic material. For example, protein-induced PSCs and chemically induced PSCs have been generated by the use of small and large (protein) molecules. Several epigenetic characteristics are important for cell differentiation; therefore, several small-molecule inhibitors of epigenetic-modifying enzymes, such as DNA methyltransferases, histone deacetylases, histone methyltransferases, and histone demethylases, are potential candidates for the reprogramming of somatic cells into iPSCs. In this review, we discuss what types of small chemical or large (protein) molecules could be used to replace the viral transduction of genes and/or genetic reprogramming to obtain human iPSCs.
AB - Induced pluripotent stem cells (iPSCs) provide a platform to obtain patient-specific cells for use as a cell source in regenerative medicine. Although iPSCs do not have the ethical concerns of embryonic stem cells, iPSCs have not been widely used in clinical applications, as they are generated by gene transduction. Recently, iPSCs have been generated without the use of genetic material. For example, protein-induced PSCs and chemically induced PSCs have been generated by the use of small and large (protein) molecules. Several epigenetic characteristics are important for cell differentiation; therefore, several small-molecule inhibitors of epigenetic-modifying enzymes, such as DNA methyltransferases, histone deacetylases, histone methyltransferases, and histone demethylases, are potential candidates for the reprogramming of somatic cells into iPSCs. In this review, we discuss what types of small chemical or large (protein) molecules could be used to replace the viral transduction of genes and/or genetic reprogramming to obtain human iPSCs.
UR - http://www.scopus.com/inward/record.url?scp=84920086707&partnerID=8YFLogxK
U2 - 10.1038/labinvest.2014.132
DO - 10.1038/labinvest.2014.132
M3 - 回顧評介論文
C2 - 25365202
AN - SCOPUS:84920086707
SN - 0023-6837
VL - 95
SP - 26
EP - 42
JO - Laboratory Investigation
JF - Laboratory Investigation
IS - 1
ER -