Functional substitution of a eukaryotic glycyl-trna synthetase with an evolutionarily unrelated bacterial cognate enzyme

Chin I. Chien, Yu Wei Chen, Yi Hua Wu, Chih Yao Chang, Tzu Ling Wang, Chien Chia Wang

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Two oligomeric types of glycyl-tRNA synthetase (GlyRS) are found in nature: a α2 type and a α2β2 type. The former has been identified in all three kingdoms of life and often pairs with tRNAGly that carries an A73 discriminator base, while the latter is found only in bacteria and chloroplasts and is almost always coupled with tRNAGly that contains U73. In the yeast Saccharomyces cerevisiae, a single GlyRS gene, GRS1, provides both the cytoplasmic and mitochondrial functions, and tRNAGly isoacceptors in both compartments possess A73. We showed herein that Homo sapiens and Arabidopsis thaliana cytoplasmic GlyRSs (both α2-type enzymes) can rescue both the cytoplasmic and mitochondrial defects of a yeast grs1- strain, while Escherichia coli GlyRS (a a2b2-type enzyme) and A. thaliana organellar GlyRS (a (αβ)2-type enzyme) failed to rescue either defect of the yeast mull allele. However, a head-to-tail ab fusion of E. coli GlyRS effectively supported the mitochondrial function. Our study suggests that a α2-type eukaryotic GlyRS may be functionally substituted with a a2b2-type bacterial cognate enzyme despite their remote evolutionary relationships.

Original languageEnglish
Article numbere94659
JournalPLoS ONE
Volume9
Issue number4
DOIs
StatePublished - 17 Apr 2014

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