Functional optical immunosensor microfluidic platform for acute myocardial infarction diagnosis

Zhi Zhong Wang, Hsiang Yu Lei, Yi Chun Chen, Chia Sheng Kuo, Can Hua Yang, Chen Han Huang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Cardiovascular disease (CVD), the leading cause of death worldwide, has been viewed as one of the major problems for wealthy and industrialized nations for decades, and the need for rapid detection and timely diagnosis has the utmost importance. Cardiac troponin I (cTnI) is a promising biomarker for early diagnosis of acute myocardial infarction (AMI). Hence, the development of immunoassay based biosensor for cTnI is necessary. Over the past decades, there have been extensive researches regarding cTnI detection, including colorimetric, fluorescence, paramagnetic, electrochemical, and surface plasmon resonance. However, conventional laboratory methods are time-consuming and require expensive and bulky equipment. In light of this, the need for point of care testing becomes more crucial. Here, we use a programmable microcontroller unit (MCU) to operate the device. A digital-to-analog converter (DAC) is used to deliver a modulating signal to LEDs, and then the modulated light excites the samples in the microfluidic reaction wells. The signals from the sample and control group are obtained by two photodetectors individually. They will be amplified and demodulated through the lock-in amplifier and digitized by analog-to-digital converters (ADC) to the MCU. And the collected data will be presented on the device and uploaded synchronically to the smartphone via Bluetooth. The whole processing time is less than 5 minutes. Next, we use the microfluidic platform to simplify complicated laboratory procedures. In our study, we focus on using cTnI to detect the samples in the human serum or blood. In order to solve low efficacy caused by the non-specific binding, we used Zwitterionic carboxybetaine disulfide (CB) as a self-assembled monolayer in the experimental design. The use of self-assembled monolayer can not only decrease non-specific binding problem but also shorten the analysis time.

Original languageEnglish
Title of host publicationMicrofluidics, BioMEMS, and Medical Microsystems XVIII
EditorsBonnie L. Gray, Holger Becker
PublisherSPIE
ISBN (Electronic)9781510632332
DOIs
StatePublished - 2020
EventMicrofluidics, BioMEMS, and Medical Microsystems XVIII 2020 - San Francisco, United States
Duration: 1 Feb 20204 Feb 2020

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11235
ISSN (Print)1605-7422

Conference

ConferenceMicrofluidics, BioMEMS, and Medical Microsystems XVIII 2020
Country/TerritoryUnited States
CitySan Francisco
Period1/02/204/02/20

Keywords

  • Cardiac troponin I
  • Cardiovascular disease
  • Microfluidic
  • Optical Immunosensor

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