Fenthion and terbufos induce DNA damage, the expression of tumor-related genes, and apoptosis in HEPG2 cells

Jong C. Wu; You Cheng Hseu; Jui Shan Tsai; Lei Chin Chen; Soi M. Chye; Chin H. Chen; Ssu Ching Chen

doi:10.1002/em.20652

Fenthion and terbufos induce DNA damage, the expression of tumor-related genes, and apoptosis in HEPG2 cells

Jong C. Wu, You Cheng Hseu, Jui Shan Tsai, Lei Chin Chen, Soi M. Chye, Chin H. Chen, Ssu Ching Chen

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

This study investigates the effects of fenthion and terbufos, two organophosphorous pesticides, on DNA damage, tumor-related gene expression, and apoptosis in HepG2 cells. We found that exposure to concentrations ranging from 50 to 200 μM of fenthion and terbufos for 2 hr caused significant death in HepG2 cells. Both compounds induced DNA damage in a concentration-dependent manner as measured using the alkaline comet assay. Tumor-related genes (jun, myc, and fos) and apoptosis-related genes (socs3, tnfaip3, ppp1r15a, and nr4a1) were up-regulated by both compounds. Finally, both compounds induced apoptosis. The results demonstrate that both terbufos and fenthion induce DNA damage and should be considered potentially hazardous to humans. Environ. Mol. Mutagen., 2011. © 2011 Wiley-Liss, Inc.

Original language	English
Pages (from-to)	529-537
Number of pages	9
Journal	Environmental and Molecular Mutagenesis
Volume	52
Issue number	7
DOIs	https://doi.org/10.1002/em.20652
State	Published - Aug 2011

Keywords

Apoptosis
Comet assay
Fenthion
Terbufos
Tumor-related genes

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title = "Fenthion and terbufos induce DNA damage, the expression of tumor-related genes, and apoptosis in HEPG2 cells",

abstract = "This study investigates the effects of fenthion and terbufos, two organophosphorous pesticides, on DNA damage, tumor-related gene expression, and apoptosis in HepG2 cells. We found that exposure to concentrations ranging from 50 to 200 μM of fenthion and terbufos for 2 hr caused significant death in HepG2 cells. Both compounds induced DNA damage in a concentration-dependent manner as measured using the alkaline comet assay. Tumor-related genes (jun, myc, and fos) and apoptosis-related genes (socs3, tnfaip3, ppp1r15a, and nr4a1) were up-regulated by both compounds. Finally, both compounds induced apoptosis. The results demonstrate that both terbufos and fenthion induce DNA damage and should be considered potentially hazardous to humans. Environ. Mol. Mutagen., 2011. {\textcopyright} 2011 Wiley-Liss, Inc.",

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AU - Wu, Jong C.

AU - Hseu, You Cheng

AU - Tsai, Jui Shan

AU - Chen, Lei Chin

AU - Chye, Soi M.

AU - Chen, Chin H.

AU - Ching Chen, Ssu

PY - 2011/8

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AB - This study investigates the effects of fenthion and terbufos, two organophosphorous pesticides, on DNA damage, tumor-related gene expression, and apoptosis in HepG2 cells. We found that exposure to concentrations ranging from 50 to 200 μM of fenthion and terbufos for 2 hr caused significant death in HepG2 cells. Both compounds induced DNA damage in a concentration-dependent manner as measured using the alkaline comet assay. Tumor-related genes (jun, myc, and fos) and apoptosis-related genes (socs3, tnfaip3, ppp1r15a, and nr4a1) were up-regulated by both compounds. Finally, both compounds induced apoptosis. The results demonstrate that both terbufos and fenthion induce DNA damage and should be considered potentially hazardous to humans. Environ. Mol. Mutagen., 2011. © 2011 Wiley-Liss, Inc.

KW - Apoptosis

KW - Comet assay

KW - Fenthion

KW - Terbufos

KW - Tumor-related genes

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JF - Environmental and Molecular Mutagenesis

IS - 7

ER -

Fenthion and terbufos induce DNA damage, the expression of tumor-related genes, and apoptosis in HEPG2 cells

Abstract

Keywords

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