Abstract
The use of inorganic nanoparticles (NPs) for biosensing requires that they exhibit high colloidal stability under various physiological conditions. Here, we report on a general approach to render hydrophobic NPs into hydrophilic ones that are ready for bioconjugation. The method uses peglyated polymers conjugated with multiple dopamines, which results in multidentate coordination. As proof-of-concept, we applied the coating to stabilize ferrite and lanthanide NPs synthesized by thermal decomposition. Both polymer-coated NPs showed excellent water solubility and were stable at high salt concentrations under physiological conditions. We used these NPs as molecular-sensing agents to detect exosomes and bacterial nucleic acids.
Original language | English |
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Pages (from-to) | 33-37 |
Number of pages | 5 |
Journal | Bioconjugate Chemistry |
Volume | 28 |
Issue number | 1 |
DOIs | |
State | Published - 18 Jan 2017 |