TY - JOUR
T1 - Fabrication and application of amperometric glucose biosensor based on a novel PtPd bimetallic nanoparticle decorated multi-walled carbon nanotube catalyst
AU - Chen, Kuan Jung
AU - Lee, Chia Feng
AU - Rick, John
AU - Wang, Shih Han
AU - Liu, Chung Chiun
AU - Hwang, Bing Joe
N1 - Funding Information:
The authors gratefully acknowledge the support from the National Science Council ( NSC-97-2120-M-011-001 and NSC-97-2221-E-011-075-MY3 ), the National Synchrotron Radiation Research Center (NSRRC) , the National Taiwan University of Science and Technology and Electronics Design Center of Case Western Reserve University .
PY - 2012/3/15
Y1 - 2012/3/15
N2 - A sensitive, selective and stable amperometric glucose biosensor employing novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) was investigated. PtPd-MWCNTs were prepared by a modified Watanabe method, and characterized by XRD and TEM. The biosensor was constructed by immobilizing the PtPd-MWCNTs catalysts in a Nafion film on a glassy carbon electrode. An inner Nafi{ligature}on film coating was used to eliminate common interferents such as uric acid, ascorbic acid and fructose. Finally, a highly porous surface with an orderly three-dimensional network enzyme layer (CS-GA-GOx) was fabricated by electrodeposition. The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062-14.07mM) and a low detection limit 0.031mM. The biosensor also showed a short response time (within 5s), and a high sensitivity (112μAmM -1cm -2). The Michaelis-Menten constant (K m) was determined as 3.3mM. In addition, the biosensor exhibited high reproducibility, good storage stability and satisfactory anti-interference ability. The applicability of the biosensor to actual serum sample analysis was also evaluated.
AB - A sensitive, selective and stable amperometric glucose biosensor employing novel PtPd bimetallic nanoparticles decorated on multi-walled carbon nanotubes (PtPd-MWCNTs) was investigated. PtPd-MWCNTs were prepared by a modified Watanabe method, and characterized by XRD and TEM. The biosensor was constructed by immobilizing the PtPd-MWCNTs catalysts in a Nafion film on a glassy carbon electrode. An inner Nafi{ligature}on film coating was used to eliminate common interferents such as uric acid, ascorbic acid and fructose. Finally, a highly porous surface with an orderly three-dimensional network enzyme layer (CS-GA-GOx) was fabricated by electrodeposition. The resulting biosensor exhibited a good response to glucose with a wide linear range (0.062-14.07mM) and a low detection limit 0.031mM. The biosensor also showed a short response time (within 5s), and a high sensitivity (112μAmM -1cm -2). The Michaelis-Menten constant (K m) was determined as 3.3mM. In addition, the biosensor exhibited high reproducibility, good storage stability and satisfactory anti-interference ability. The applicability of the biosensor to actual serum sample analysis was also evaluated.
KW - Glucose amperometric sensor
KW - Hydrogen peroxide sensing
KW - Pt-based bimetallic nanoparticles (NPs)
KW - PtPd NPs-decorated multi walled carbon nanotube catalyst
UR - http://www.scopus.com/inward/record.url?scp=84862818150&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2011.12.020
DO - 10.1016/j.bios.2011.12.020
M3 - 期刊論文
C2 - 22277115
AN - SCOPUS:84862818150
SN - 0956-5663
VL - 33
SP - 75
EP - 81
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 1
ER -