Extracellular electrons transferred from honey probiotic Bacillus circulans inhibits inflammatory acne vulgaris

Hsin Jou Kao, Arun Balasubramaniam, Chun Chuan Chen, Chun Ming Huang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Bacillus circulans (B. circulans) is widely used as an electrogenic bacterium in microbial fuel cell (MFC) technology. This study evaluated whether B. circulans can ferment glucose to generate electricity and mitigate the effects of human skin pathogens. The electricity production of B. circulans was examined by measuring the voltage difference and verified using a ferrozine assay in vitro. To investigate the fermentation effects of B. circulans on inhibition of human skin pathogens, Cutibacterium acnes (C. acnes) was injected intradermally into mice ears to induce an inflammatory response. The results revealed that the glucose–B. circulans co-culture enhanced electricity production and significantly supressed C. acnes growth. The addition of roseoflavin to inhibit flavin production considerably reduced the electrical energy generated by B. circulans through metabolism and, in vivo test, recovered C. acnes count and macrophage inflammatory protein 2 (MIP-2) levels. This suggests that B. circulans can generate electrons that affect the growth of C. acnes through flavin-mediated electron transfer and alleviate the resultant inflammatory response. Our findings demonstrate that probiotics separated from natural substances and antimicrobial methods of generating electrical energy through carbon source fermentation can help in the treatment of bacterial infections.

Original languageEnglish
Article number19217
JournalScientific Reports
Issue number1
StatePublished - Dec 2022


Dive into the research topics of 'Extracellular electrons transferred from honey probiotic Bacillus circulans inhibits inflammatory acne vulgaris'. Together they form a unique fingerprint.

Cite this