Expression of a gene encoding a rice RING zinc-finger protein, OsRZFP34, enhances stomata opening

Kuo Hsuan Hsu, Chia Chin Liu, Shaw Jye Wu, Ying Yu Kuo, Chung An Lu, Ching Rong Wu, Pei Jyun Lian, Chwan Yang Hong, Yi Ting Ke, Juin Hua Huang, Ching Hui Yeh

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

By oligo microarray expression profiling, we identified a rice RING zinc-finger protein (RZFP), OsRZFP34, whose gene expression increased with high temperature or abscisic acid (ABA) treatment. As compared with the wild type, rice and Arabidopsis with OsRZFP34 overexpression showed increased relative stomata opening even with ABA treatment. Furthermore, loss-of-function mutation of OsRZFP34 and AtRZFP34 (At5g22920), an OsRZFP34 homolog in Arabidopsis, decreased relative stomata aperture under nonstress control conditions. Expressing OsRZFP34 in atrzfp34 reverted the mutant phenotype to normal, which indicates a conserved molecular function between OsRZFP34 and AtRZFP34. Analysis of water loss and leaf temperature under stress conditions revealed a higher evaporation rate and cooling effect in OsRZFP34-overexpressing Arabidopsis and rice than the wild type, atrzfp34 and osrzfp34. Thus, stomata opening, enhanced leaf cooling, and ABA insensitivity was conserved with OsRZFP34 expression. Transcription profiling of transgenic rice overexpressing OsRZFP34 revealed many genes involved in OsRZFP34-mediated stomatal movement. Several genes upregulated or downregulated in OsRZFP34-overexpressing plants were previously implicated in Ca2+ sensing, K+ regulator, and ABA response. We suggest that OsRZFP34 may modulate these genes to control stomata opening.

Original languageEnglish
Pages (from-to)125-137
Number of pages13
JournalPlant Molecular Biology
Volume86
Issue number1-2
DOIs
StatePublished - Sep 2014

Keywords

  • ABA
  • Heat stress
  • Rice
  • RING zinc finger protein
  • Stomata
  • Transpiration

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