Epigallocatechin-3-gallate Reduces Scavenger Receptor A Expression and Foam Cell Formation in Human Macrophages

Sy Jou Chen, Yung Hsi Kao, Li Jing, Yi Ping Chuang, Wan Lin Wu, Shu Ting Liu, Shih Ming Huang, Jenn Haung Lai, Ling Jun Ho, Min Chien Tsai, Chin Sheng Lin

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Foam cells are formed when macrophages imbibe low-density lipoprotein (LDL) through scavenger receptors. Here we examined how epigallocatechin-3-gallate (EGCG) influences foam cell formation. We found that EGCG dose-dependently reduced oxidized LDL (oxLDL) uptake in THP-1 (10 μM, 20.0 ± 0.50, p < 0.05) and primary macrophages (134.6 ± 15.6, p < 0.05) and reduced intracellular cholesterol content in these cells, respectively (10 μM, 32.6 ± 0.14, p < 0.05; 31.7 ± 1.26, p < 0.05). EGCG treatment decreased scavenger receptor A expression, but not the expression of CD36 or of reverse cholesterol transporters. Moreover, EGCG stimulated translocation of the p50 and p65 subunits of NF-κB and enhanced NF-κB DNA-binding activity, thus suppressing SR-A promoter activity. EGCG's suppression of SR-A expression was blocked by the NF-κB inhibitor Bay. The present findings suggest that EGCG regulates NF-κB activity and thus suppresses SR-A expression, oxLDL uptake, and foam cell formation.

Original languageEnglish
Pages (from-to)3141-3150
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume65
Issue number15
DOIs
StatePublished - 19 Apr 2017

Keywords

  • atherosclerosis
  • epigallocatechin-3-gallate
  • foam cells
  • macrophage
  • oxidized low-density lipoprotein

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