Enzymatic determination of diglyceride using an iridium nano-particle based single use, disposable biosensor

Shu Yi Hsu; Brandon Bartling; Christina Wang; Fuh Sheng Shieu; Chung Chiun Liu

doi:10.3390/s100605758

Enzymatic determination of diglyceride using an iridium nano-particle based single use, disposable biosensor

Shu Yi Hsu, Brandon Bartling, Christina Wang, Fuh Sheng Shieu, Chung Chiun Liu

Graduate Institute of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 μM in phosphate buffer and bovine serum media.

Original language	English
Pages (from-to)	5758-5773
Number of pages	16
Journal	Sensors (Switzerland)
Volume	10
Issue number	6
DOIs	https://doi.org/10.3390/s100605758
State	Published - Jun 2010

Keywords

Diglyceride
Disposable biosensor
Enzyme

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abstract = "A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 μM in phosphate buffer and bovine serum media.",

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AU - Bartling, Brandon

AU - Wang, Christina

AU - Shieu, Fuh Sheng

AU - Liu, Chung Chiun

PY - 2010/6

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N2 - A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 μM in phosphate buffer and bovine serum media.

AB - A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 μM in phosphate buffer and bovine serum media.

KW - Diglyceride

KW - Disposable biosensor

KW - Enzyme

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Enzymatic determination of diglyceride using an iridium nano-particle based single use, disposable biosensor

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Keywords

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