Projects per year
Abstract
Super-resolution imaging based on single-molecule localization microscopy combined with the surface plasmon polariton (SPP)-enhanced fluorescence of spontaneously blinking fluorophores was demonstrated to visualize the nanoscale-level positioning information of cell-adhesion-associated proteins. Glass substrates with a deposited silver layer were utilized to induce a SPP-enhanced field on the silver surface and significantly strengthen the fluorescence signals of the fluorophores by more than 300%. The illumination power density for localization imaging at a spatial resolution of 25 ± 11 nm was 31.6 W cm-2. This low illumination power density will facilitate the reduction of phototoxicity of the biospecimens for single-molecule localization imaging. The proposed strategy provides a uniform distribution of the SPP-enhanced field on the silver surface, enabling visualization of the spatial distribution of labeled proteins without interference caused by the enhanced field distribution.
Original language | English |
---|---|
Pages (from-to) | 27245-27255 |
Number of pages | 11 |
Journal | Physical Chemistry Chemical Physics |
Volume | 20 |
Issue number | 43 |
DOIs | |
State | Published - 2018 |
Fingerprint
Dive into the research topics of 'Enhancing the blinking fluorescence of single-molecule localization imaging by using a surface-plasmon-polariton-enhanced substrate'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Super-Resolution Imaging Informatics for Molecule Functions Analysis in Thick Biological Specimens(2/3)
Chien, F.-C. (PI)
1/08/17 → 31/07/18
Project: Research