We present a novel method to enhance the DNA hybridization efficiency by photovoltaic effect. The arrayed microchip is fabricated in a semiconductor wafer coated with metal thin films for immobilizing the probe DNA. Hybridization in between immobilized probe DNAs and fluorescent-labeled target DNA are carried out through the illumination of a laser. Photovoltaic effect induces positive charges on the surface of microchip to attract negatively charged target DNAs down to the hybridization site, results in efficiently enhancing the hybridization rate up to two-order magnitude. Our observation provided cutting-edge solution away from bottleneck of microchip process in heat and time-consuming.
|Title of host publication
|CLEO/Pacific Rim 2003 - 5th Pacific Rim Conference on Lasers and Electro-Optics
|Subtitle of host publication
|Photonics Lights Innovation, from Nano-Structures and Devices to Systems and Networks, Proceedings
|Institute of Electrical and Electronics Engineers Inc.
|Number of pages
|Published - 2003
|5th Pacific Rim Conference on Lasers and Electro-Optics, CLEO/Pacific Rim 2003 - Taipei, Taiwan
Duration: 15 Dec 2003 → 19 Dec 2003
|Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest
|5th Pacific Rim Conference on Lasers and Electro-Optics, CLEO/Pacific Rim 2003
|15/12/03 → 19/12/03