Enhanced production of antigen (CEA) and human antibody by mammalian cells cultured on various polymeric films

Shin Adachi, Mariko Hara, Masanori Kamei, Shuichi Hashizume, Akon Higuchi

Research output: Contribution to journalConference articlepeer-review

Abstract

The growth and production of carcinoembryonic antigen (CEA) were studied for human colorectal adenocarcinoma tumor (CW2) cells cultured with caffeine, ethylene glycol, and thymidine, as well as on PMLG, PBLG, silk fibroin, and collagen films. CW2 cells produced CEA by a factor of more than 5-fold by addition of caffeine to the cell culture media after 13 days of cultivation. CW2 cells did not enhance the production of CEA by the addition of thymidine into the cell culture media. The CW2 cells produced CEA efficiently when the cells were cultured on the films where the serum proteins adsorbed with native secondary conformation.

Original languageEnglish
Pages (from-to)1693-1694
Number of pages2
JournalAmerican Chemical Society, Polymer Preprints, Division of Polymer Chemistry
Volume41
Issue number2
StatePublished - Aug 2000
EventWashington,DC. Meeting - Washington, DC, USA
Duration: 20 Aug 200024 Aug 2000

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