Electrochemical evaluation of glucose oxidase immobilized by different methods

E. J. Lahoda, C. C. Liu, L. B. Wingard

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Glucose oxidase electrodes were constructed on a platinum screen using polyacrylamide gel, glutaraldehyde crosslinking, and glutaraldehyde crosslinking with +0.04 volts dc on the platinum screen as the methods of enzyme immobilization. The electrodes were evaluated in an electrochemical cell for the oxidation of glucose at the enzyme electrode and the reduction of oxygen at a platinum auxiliary electrode, using constant current voltametry or under external load operation. The method of immobilization affected the extrapolated opencircuit potential as well as the half‐cell potential and the steady current under external load operation. The charged glutaraldehyde electrode gave the best current performance; however, the small output (microamps) indicated that major problems in electron transfer from an enzyme catalyst to an external circuit must be resolved before such electrodes can be used in practical application.

Original languageEnglish
Pages (from-to)413-422
Number of pages10
JournalBiotechnology and Bioengineering
Volume17
Issue number3
DOIs
StatePublished - Mar 1975

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