Effects of light quality on the accumulation of oil in a mixed culture of Chlorella sp. and Saccharomyces cerevisiae

Chin Hang Shu, Chieh Chung Tsai, Wei Hsiu Liao, Kun Yan Chen, Hao Chen Huang

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

Background: Chlorella strains rather than terrestrial oil crops having higher oil content and shorter generation time have been considered as promising candidates for alternative biodiesel. Since the influence of light quality on oil formation of microalgae in either monoculture or mixed culture has been shown to be either inconsistent or ambiguous, a light-emitting diode (LED) photo-bioreactor with different light sources and intensities was used in this study to investigate a cost-effective lipid production process. Results: The oil accumulation in a mixed culture of Chlorella sp. and Saccharomyces cerevisiae was higher than that in the monoculture under the different light sources used. Results of the influence of light quality on the mixed culture indicated that the optimal light wavelength and intensity for biomass formation was red LED light at 1000 lux, whereas the optimum for oil formation was blue LED light at 1000 lux. A novel two-stage LED photo-bioreactor was thus proposed and the highest P max and productivity in this study were obtained as 261 mg L -1 and 8.16 mg L -1 h -1, respectively. Conclusion: A novel two-stage LED photo-bioreactor using a mixed culture to optimize microalgal oil production was proposed and successfully demonstrated in this study.

Original languageEnglish
Pages (from-to)601-607
Number of pages7
JournalJournal of Chemical Technology and Biotechnology
Volume87
Issue number5
DOIs
StatePublished - May 2012

Keywords

  • Chlorella sp.
  • LED photo-bioreactor
  • Light quality
  • Lipid
  • Mixed-culture

Fingerprint

Dive into the research topics of 'Effects of light quality on the accumulation of oil in a mixed culture of Chlorella sp. and Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this