Effects of culture pH on the production of bioactive polysaccharides by Agaricus blazei in batch cultures

Chin Hang Shu, Ko Jung Lin, Bor Jiun Wen

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The effects of culture pH on the production, molecular weight distribution and the bioactivity of polysaccharides produced by Agaricus blazei were evaluated by four pH-controlled batch cultures. As the culture pH of each batch was controlled from 4.0 to 7.0, the maximum polysaccharide concentration increased from 561 to 1252 mg dm-3, but the average molecular weight of the polysaccharides decreased monotonically from 1080 kDa to 600 kDa, the relative amount of β-glucan from 56% to 33%, and the TNF-α release by macrophage cells from 1440 to 760 pg per 5 × 104 cells. Polysaccharides with higher molecular weights, β-glucan contents and bioactivities were obtained in the submerged cultures of Agaricus blazei at lower culture pH but with lower yields.

Original languageEnglish
Pages (from-to)998-1002
Number of pages5
JournalJournal of Chemical Technology and Biotechnology
Volume79
Issue number9
DOIs
StatePublished - Sep 2004

Keywords

  • β-glucan
  • Agaricus blazei
  • Molecular weight
  • pH
  • Polysaccharide
  • Tumor necrosis factor-α

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